Photorhabdus luminescens

Escherichia coli K12 TGI strain was used as a recipient for transformation. At studying of SOS-system activity the recombinant bioluminescent strain of Escherichia coli recA lux containing plasmid-borne fusions of the recA promoter-operator region to the Photorhabdus luminescens ZM 1 lux genes (GosNlIgenetika, Russia) was used. Increase of their luminescence in the presence of DNA damage factors [Rosen et al., 2000], were shown previously. Investigation of the luminescent response of this strain to UV radiation allows quantitatively estimate in a real time a SOS-system induction. 

Ehlers RU, Oestergaard J, Hollmer S, Wingen M, Strauch O. Genetic selection for heat tolerance and low temperature activity of the entomopathogenic nematode-bacterium complex Heterorhabditis bacteriophora - Photorhabdus luminescens. Bio Cont (Dordrecht). 2005 50 699-716. 

Mahar AN, Jan ND, Mahar AQ, Mahar GM, Hullio MH, Lajar AG. Efficacy of entomopathogenic bacterium Photorhabdus luminescens and its metabolites against diamondback moth Plutella xylostella larvae on Chinese cabbage and artificial diet. Pak J Nematol. 2008 26 69-82. 

Wang Y, Bilgrami AL, Shapiro-Ilan D, Gaugler R. Stability of entomopathogenic bacteria, Xenorhabdus nematophila and Photorhabdus luminescens, during in vitro culture. J Indus Micro Biotech. 2007 34 73-81. 

To this group of siderophores belong photobactin (42a) from Photorhabdus luminescens (Fig. 12) 66), derived from 1,4-diaminobutane substituted by DHB and by cyclized DHB-Thr ( H-NMR data indicate that the substituents of the oxazoline ring are in trans positions the absolute stereochemistry is not known),... 

Bioluminescence serves as an excellent reporter system as a sensitive marker for microbial detection, as a real-time, non-invasive reporter for measuring gene expression and as a measure of intracellular biochemical function (cell viability). Most widely studied of the bioluminescence systems are those belonging to the luminous bacteria Vibrio sp.. Photobacterium sp. and Photorhabdus luminescens) and the firefly Photinus pyralis). While these systems have proved extremely versatile, there are caveats to their use limiting the array of applications they can be applied to. These caveats mainly surround the nature of the luciferase enzymes, and include temperature and pH stability. 

Chen, G., Dunphy, G.B. and Webster, J.M. (1994) Antifungal activity of two Xenorhab-dus species and Photorhabdus luminescens, bacteria associated with the nematodes Steinernema species and Heterorhabditis megidis. Biological Control 4, 157-162. 

PAL and TAL are apparently represented to a very limited extent in bacteria, this presumably accounting for the general paucity of phenylpropanoids in such organisms. Isolation and characterization of PALs have, however, been described for R. toruloides Streptomyces maritimus Photorhabdus luminescens Sorangium cdlulosum, and Streptomyces verticillatus with the -cinnamic acid (3) so-formed being considered, for... 

Photorhabdus luminescens A soru ce of natmal insecticides to complement or substitute for the toxin genes from Bacillus thuringiensis. Genomic analysis should yield function-nal infomration to enable the engineering of more potent and specific toxins. 

Winson, M. K., Swift, S., Hill, P. J., Sims, C. M., Griesmayr, G., Bycroft, B. W., Williams, P., and Stewart, G. S. (1998) Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs. FEMS Microbiol. Lett. 163, 193-202. 

O-polysaccharide Photorhabdus luminescens subsp. laumondii TTOl 262... 

Kino K, et al. (2010) Identification and Characterization a Novel L-Amino Acid Ligase from Photorhabdus luminescens subsp. laumondii TTOl. J. biosci. bioengin. 110 p. 39-41. 

This hypothesis was tested by another group for a previously uncharacterized ER from Photorhabdus luminescens subsp laumondii identified... 

Abbreviations for cell strains are as follows Vh, Vibrio harveyi Vf, Vibrio fischeri Plu, Photorhabdus luminescens. Pie, Photobacterium leiognathi Pp, Photobacterium phosphoreum. The bacterium strain is followed by the designation of the luciferase subunit. Active luciferase heterodimers are indicated by ++ for the native enzymes and + for the hybrid enzymes. Inactive hybrids are indicated by the negative sign. 

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