Vectors cosmids

Cosmetics industry Cosmic radiation Cosmid vectors Cosmocil CQ CoS04 7H20 Cosorb process Cost estimates... 

M Inouye, Y Takada, N Muto, T Beppu, S Horinouchi. Characterization and expression of a P-450-like mycinamicin biosynthesis gene using a novel Micromonospora-Escherichia coli shuttle cosmid vector. Mol Gen Genet 245 456-464, 1994. 

Kuhstoss, S., M. Huber, J. R. Turner, J. W. Paschal, R.N. Rao (1996). A new shuttle cosmid vector, pKC505, for streptomycetes its use in the cloning of three different spiramycin-resistance genes from a Streptomyces ambofaciens library. Gene 183 231-241. 

S., and Sano, K. (1985) Construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria Brevibacterium lactofermen-tum and Corynebacterium glutamicum. Gene, 39 (2-3), 281-286. 

Chromosomal localization of the axl gene is done by fluorescence in situ hybridization (FISH). The biotin-labeled axl probe is from a cosmid vector containing a 41-kb genomic fragment. The slides with human metaphase cells for chromosomal DNA to be denatured are immersed into a solution of 70 % formamide-x4 in saline sodium citrate buffer (SSC) atpH 7.0 (0.15 M NaCl 0.015 M sodium citrate) at 70 °C for 2 min. For the slides, the hybridization mixture kept at 75 °C consists of 50 %formamide,xl SSC, 10 % dextran sulfate pH 7.0, and 150 mL unlabeled sonicated total human genomic DNA add 50-100 ng labeled probe. The mixture is incubated at 37 °C for 10 min. The slides are washed in 50 % formamide and SSC x3, 5 min each at 40 °C and in SSC x3 at 40 °C. The detection reagent consists of x4 SSC-0.1 % Triton, and 1 % bovine semm albumin for 3 washes 3 min each at 40 °C. The slides are incubated with fluorescein isothiocyanate-conjugated avidin at 37 °C for 30 min. Metaphase cells are counterstained with 4,6-diamidino-2-phenylindole dihydrochloride 200 ng/mL in 2x SSC for 5 min at... 

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