Escherichia coli expression

Rodriguez, S., Schroeder, K.T., Kayser, M.M. and Stewart, J.D. (2000) Asymmetric synthesis of /3-hydroxy esters and alpha-alkyl-beta-hydroxy esters by recombinant Escherichia coli expressing enzymes from baker s yeast. The Journal of Organic Chemistry, 65 (8), 2586-2587. 

Parikh, A., Gillam, E.M. and Guengerich, F.P. (1997) Drug metabolism by Escherichia coli expressing human cytochromes P450. Nature Biotechnology, 15, 784—788. 

Steiner TS, Nataro JP, Poteet-Smith CE, Smith JA, Guerrant RL Enteroaggregative Escherichia coli expresses a novel fiagellin that causes IL-8 release from intestinal epithelial cells. J Clin Invest 2000 105 1769-1777. 

Anakinra is a nonglycosylated form of the human IL-1 receptor antagonist (IL-lra). It is produced in a recombinant Escherichia coli expression system and has an additional methionine residue at its amino terminus. In rheumatoid arthritis patients, the amount of naturally occurring IL-lra in the synovial fluid is not sufhcient to counteract the high levels of locally produced IL-1. Anakinra acts as a competitive antagonist of the type 1 IL-1 receptor and decreases the pain and inflammation produced by IL-1. It is administered as a daily subcutaneous injection. 

Actimmune (interferon-gamma-1 b) [Escherichia coli expressed InterMune Liquid (single-dose vial) lOOpg in 0.5 mL 0.2mg/mL Per 0.5 mL 20 mg mannitol 0.36mg sodium succinate 0.05 mg polysorbate 20 SC... 

Nakamura, K., Iwasaki, Y. Hattori, T. (1980). An improved Escherichia coli expression vector for the construction and identification of full-length cDNA clones. Gene 44, 347-51. 

Pliickthun, A. (1991) Antibody engineering advances from the use of Escherichia coli expression systems. Bio/Technology 9,545-551. 

Strong, L.C., H. McTavish, M.J. Sadowsky, and L.P. Wackett (2000). Field-scale remediation of atrazine-contaminated soil using recombinant Escherichia coli expressing atrazine chlorohydrolase. Environ. Microbiol., 2 91-98. 

Maruyama, N., Ichise, K., Katsube, T., Kishimoto, T., Kawase, S., Matsumura, Y., Takeuchi, Y., Sawada, T., Utsumi, S. 1998. Identification of major wheat allergens by means of Escherichia coli expression system. EurJBiochem 255 739-745. 

In an Escherichia coli expression system for the aqualysin I precursor, the precursor is processed autoproteolytically into the mature 28-kDa enzyme by treatment at 65 ° C.23) In this case, the N-terminal pro-sequence is required for the production of active enzyme and functions to stabilize the precursor structure.283 The C-terminal pro-sequence is not essential for the production of active aqualysin 1,293 but seems to be involved in the translocation of the precursor across the cytoplasmic membrane.303 In a Thermus thermophilus expression system,313 the C-terminal pro-sequence is required for the production and extracellular secretion of active aqualysin I.323 In an E. coli expression system for the subtilisin E gene, the N-terminal pro-sequence is essential for the production of active enzyme,333 as in the case of aqualysin I. The requirement of the pro-sequence is also shown in vitro for the refolding of the inactive mature protein to an active enzyme.34 353 The functions of the N-terminal pro-sequences of aqualysin I and subtilisin E seem to be similar. 

Francisco, J. A., Campbell, R., Iverson, B. L., and Georgiou, G. (1993). Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. Proc. Natl. Acad. Sci. USA 90, 10444—10448. 

Francisco JA, Stathopoulos C, Warren RAJ, Kilburn DG, Georgiou G, Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains, Biotechnology, 11 491 495, 1993. 

Robertson MW, Phage and Escherichia coli expression of the human high affinity immunoglobulin E receptor alpha-subunit ectodomain, Domain localization of the IgE-binding site, J. Biol. Chem., 268 12736-12743, 1993. 

Miki Y, Morales M, Ruiz-Duenas FJ et al (2009) Escherichia coli expression and in vitro activation of a unique ligninolytic peroxidase that has a catalytic tyrosine residue. Protein Express Purif 68 208-214... 

Rec. Escherichia coli expressing Saccharomyces cerevisia glucose-6-phosphate dehydrogenase... 

F3. Freije, J. P., Balbin, M., Abrahamson, M., Velasco, G., Dalb0ge, H., et al., Human cystatin D. cDNA cloning, characterization of the Escherichia coli expressed inhibitor, and identification of the native protein in saliva. J. Biol. Chem, 268(21), 15737-15744 (1993). 

Kim, B.G., Lee, Y., Hur, H.G., Lim, Y., Ahn, J.H. (2006b) Production of three O-methylated esculetins with Escherichia coli expressing O-methyltransferases from poplar. Biosci., Biotechnol. Biochem., 70,1269-72. 

A breakthrough in the study of HK97 was achieved when an Escherichia coli expression system was developed in which gene 4 and gene 5 products alone were shown to assemble and mature in a manner identical with the authentic virus (Xie and Hendrix, 1995). Expansion was triggered by... 

Pinkney M, Kapur V, Smith J et al. (1995) Different forms of streptolysin O produced by Streptococcus pyogenes and by Escherichia coli expressing recombinant toxin cleavage by streptococcal cystein protease. In Infect Immun 63 2776-2779. 

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