Eosinophil chemoattractant

P9. Ponath, P. D., Qin, S., Ringler, D. I., Clark-Lewis, I., Wang, I., Kassam, N., Smith, H., Shi, X., Gonzalo, J. A., Newman, W., Gutierrez-Ramos, I. C., and Mackay, C. R., Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J. Clin. Invest. 97, 604-612... 

Erlemann, K.R., Rokach, J., and Powell, W.S., Oxidative stress stimulates the synthesis of the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid by inflammatory cells, J. Biol. Chem., 2004, in press. 

Weber, M Uguccioni, M., Baggiolini, M Clark-Lewis, I., andDahinden, C. A. (1996) Deletion of the NH2-terminal residue converts monocyte chemotactic protein 1 from an activator of basophil mediator release to an eosinophil chemoattractant. J. Exp. Med. 183, 681-685. 

In this chapter, we describe the radioactive and enzymic methods used to measure the accumulation of eosinophils in guinea pig skin. The mIn-labeled cell method provides results on the same day as the in vivo assay. This is especially useful when monitoring activity in HPLC fractions during purification of eosinophil chemoattractants because the speed of the assay facilitates progression to the next stage of purification. Although the EPO method takes at least one extra day to obtain results, it has the advantage that the eosinophils measured have not been subjected to isolation procedures which can modify their response to chemoattractant mediators. We first describe the isolation and purification of the eosinophils required for both methods as a source of cells to radiolabel and to provide a standard curve for the EPO assay. We then describe the mIn-labeled cell and EPO methods. 

Zhu J, Qiu YS, Majumdar S, Gamble E, Matin D, Turato G, Fabbri LM, Barnes N, Saetta M, Jeffery PK. Exacerbations of Bronchitis Bronchial eosinophilia and gene expression for interleukin-4, interleukin-5, and eosinophil chemoattractants. Am. J. Respir. Crit. Care Med. 2001 164 109-116. 

Cruikshank, W., Melissa, F., Teran, L. and Center, D. (1994a). Early detection of a CD4 lymphocyte and eosinophil chemoattractant in bronchoalveolar lavage fluid from asthmatics following antigen challenge. Am. Thor. Soc. 149, A954. 

IL-16 T cells mast cells eosinophils CD4 T cells chemoattractant monocytes chemoattractant eosinophils chemoattractant T cells, anti-apoptotic for IL-2-activated cells... 

SHUTE, J. (1994) Interleukin-8 is a potent eosinophil chemoattractant. Clinical and Experimental Allergy, 24, 203-206. 

JOSE, P.H., GRIFFITHS-JOHNSON, D.A., COLLINS, P.D., WALSH, D.T., MOQBEL, R TOTTY, N.F., TRUONG, O., HSUAN, J.J. WILLIAMS, TJ. (1994) Eotaxin a potent eosinophil chemoattractant cytokine detected in a guinea pig model of allergic airways inflammation. Journal of Experimental Medicine, 179, 881-887. 

WANG, J.M., RAMBALDI, A., BIONDI, A., CHEN, Z.G., SANDERSON, CJ. MANTOVANI, A. (1989) Recombinant interleukin 5 is a selective eosinophil chemoattractant. European Journal of Immunology, 19, 701-705. 

Rothenberg, M.E., Luster, A.D., and Leder, P. (1995) Murine eotaxin An eosinophil chemoattractant inducible in endothelial cells and in interleukin 4-induced tumor suppression. Proceedir ... 

Other CC chemokines are likely also important in the development of eosinophilic airway inflammation. MCP-3 (MARC/FIC) was originally isolated from mast cells, which would immediately center a role for this eosinophil chemoattractant on early allergic responses. Depletion of MCP-3 in allergic airway models has demonstrated a significant role for this molecule on eosinophil recruitment to the airways (39). In addition, studies in our own laboratory examining the role of other CC chemokines, specifically MIP-la, have demonstrated that it is not only important in mononuclear recruitment... 

---