Enzymatic Activity and Effects on Cellular Lipid Metabolism of Endothelial Lipase

Enzymatic Activity and Effects on Cellular Lipid Metabolism of Endothelial Lipase 

EL can hydrolyze both triglyceride and phosphohpids. In fhe initial reports on this enzyme, two groups reported the ability of EL in conditioned medium to hydrolyze phosphatidylcholine. Hirata et al. originally reported an inability of EL to hydrolyze triglyceride [4], whereas Jaye et al. reported fhat EL hydrolyzed a radiolabeled triolein substrate, albeit at a low level [3]. 

McCoy et al. also compared EL to LPL and HL in their substrate preferences [16]. Using lipases expressed by adenoviral gene transfer into Cos-1 cells, fhe tri- 

In a manner similar to LPL inhibition, EL triglyceride lipase and phospholipase activities were completely abolished in the presence of 1 m NaCl. 

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