Embryo fate mapping

Lohs-Schardin M., Sander K., Cremer C., Cremer T, and Zorn C. 1979b. Localized ultraviolet laser microbeam irradiation of early Drosophila embryos Fate maps based on location and frequency of adult defects. Dev. Biol. 68 533—545. 

Arora K, Niisslein-Volhard C 1992 Altered mitotic domains reveal fate map changes in Drosophila embryos mutant for zygotic dorsoventral patterning genes. Development 114 1003-1024... 

Fate maps only reveal contributions of cells in different regions but do not show when decisions to form a particular structure have been taken. Once cells have taken this decision, which seals their fate irreversibly, they are said to be determined and the traditional test for determination is transplantation. If cells are determined, they will develop autonomously when transplanted into a different region of an embryo if they are not, they will change fate and develop according to their new site (Slack, 1991). 

Couly, G., LeDouarin, N. 1988. The fate map of cephalic neural primordium at the presomitic to the 3-somtie stage in the avian embryo. Development 103 Suppl., 101-113. 

Fig. 12.1. Fate map of an avian embryo before the beginning of gastrulation.

Serbedzija GN, Bronner-Fraser M, Fraser SE (1989) A vital dye analysis of timing and pathways of avian neural crest cell migration. Development 106 809-816. Kinder SJ, Tan S-S, Tam PPL (2000). Cell grafting and fate mapping of the early-somite-stage mouse embryo. In Methods in molecular biology, vol.l, pp. 425 37, Developmental Biology Protocols, vol. 135. 

Catala, M., Teillet, M.-A., De Robertis, E. M., and Le Douarin, N. M. (1996) A spinal cord fate map in the avian embryo while regressing, Hensen s node lays down the notochord and floor plate thus joining the spinal cord lateral walls. 

Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo... 

Labeling single cells in ovo and then studying their fate during subsequent development can be a powerful tool in the investigation of how embryos develop. It can be used to construct fate maps at the single-cell level, analyze... 

After the fourth (meridional) and fifth (equatorial) cleavages, the embryo comprises 32 cells, arranged in four tiers of eight cells (stage 6 of Nieuwkoop and Faber). The fates of these cells have been determined by microinjection of cell lineage markers (8,9), and the fate map of Dale and Slack is shown in Fig. 2. Notice that individual cells at this stage are far from restricted to a single fate, and that there may be considerable variation from embryo to embryo. 

Tavian M, Robin C, Coulombel L and Peault B (2001). The human embryo, but not its yolk sac, generates lympho-myeloid stem cells, mapping multipotent hematopoietic cell fate in intraembryonic mesoderm. Immunity 15 487-495. 

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