Embryo cells from mouse

Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292 154—156... 

Evans, MJ. Kaufman, M. Establishment in cultme of pluripotential stem cells from mouse embryos. Nature 292,151 156 (1981). 

Hatzopoulos AK, Folkman J, Vasile E, Eiselen GK, Rosenberg RD. Isolation and characterization of endothelial progenitor cells from mouse embryos. Development 1998 125 1457-1468. 

The presence in cellular systems of conjugating enzymes may partially explain some of the differences which have been observed in the nature of BaP-DNA adducts when microsomal versus cellular systems were used for activation. While the major adducts formed in rodent embryo cells or mouse skin resulted from reaction of BaP-7,8-diol-9,10-epoxide, isomer I, with DNA, another adduct was formed from BaP in the presence of rat liver microsomes 238, 271), This adduct appears to result from reaction of DNA with the 4,5-epoxide of 9-hydroxy-BaP. The factors controlling formation of this adduct are not completely understood, since it has not always been observed when microsomal systems were used for BaP activation 444). 

Figure 2. Computed kinetics of water loss from mouse ova cooled at 1 °C to 32 °C/min in 1M DMSO. The curve labeled EQ shows the water content that ova have to maintain to remain in equilibrium with extracellular ice. If ova or embryos contain more than equilibrium amounts of water when they cool to below -30 °C, they will undergo intracellular freezing. Usually such freezing is lethal, but if the quantity of ice is small, some internally frozen cells can be rescued by rapid warming. (From Mazur, 1990.)...

Rawles, M. E. (1947). Origin of pigment cells from the neural crest in the mouse embryo. Physiol. Zool. 20 248-266. 

Cuthbertson KS, Whittingham DG, Cobbold PH 1981 Free Ca2+ increases in exponential phases during mouse oocyte activation. Nature 294 754—757 Day ML, Johnson MH, Cook DI 1998 A cytoplasmic cell cycle controls the activity of a K+ channel in pre-implantation mouse embryos. EMBO J 17 1952—1960 Flach G, Johnson MH, Braude PR, Taylor RA, Bolton VN 1982 The transition from maternal to embryonic control in the 2-cell mouse embryo. EMBO J 1 681-686 Howlett SK 1986 A set of proteins showing cell cycle-dependent modification in the early mouse embryo. Cell 45 387-396... 

From observations with the electron microscope, it was suggested that sulfation of the carbohydrate moieties occurs on the endoplasmic reticulum or in the Golgi apparatus.359,360 The finding of sulfotrans-ferase (E.C. 2.8.2) activity in microsomal fractions from both chick-embryo epiphyses350 and mouse, mast-cell tumor361 may be supporting evidence. 

Manipulation of mouse teratoma cells. The cells from a teratocarcinoma may be dispersed and grown in tissue culture. These cells can be injected into an embryo (a), in which case the resulting animal is a chimera in which some cells come from the original parents and others arise from the cells injected into the blastocyst. 

Figure 3. Time course of incorporation of ssSOi>" into cerebroside sulfate (cer-SOk) and sulfogalactosyl glycerol lipid (SGG-lipid) in dissociated brain cells from 15-day mouse embryos grown 19 days in culture...

Pretazettine (395) has been the subject of numerous biological studies, and it has been shown to exhibit a number of interesting activities (96,97,101,178-187). For example, 395 was found to inhibit HeLa cell growth as well as protein synthesis in eukaryotic cells by interfering with the peptide bond formation step (97,101). Furthermore, pretazettine inhibited the purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus, a typical C-type virus (178), in an unusual fashion since it physically combined with the polymerase enzyme itself rather than interacted with the nucleic acid template. Pretazettine also exhibited antiviral activity against the Rauscher leukemia virus in mouse embryo cell cultures by suppressing viral replication (179). 

Lee J, Inoue K, Ono R, Ogonuki N, Kohda T, Kaneko-Ishino T, Ogura A, Ishino F. Erasing genomic imprinting memory in mouse clone embryos produced from day 11.5 primordial germ cells. Development 2002 129 1807— 1817. 

Figure 2. Sephadex LH-20 column chromatography of DMBA-deoxyribo-nucleoside adducts formed by enzymatic digestion of DNA from mouse embryo cells exposed to [l Cj-DMBA (0.2 yg/ml) for 24 h ( - ) and of DNA from the skin of female NIH Swiss mice treated with [3h]-DMBA (10 yg/mouse) for 24 h (0-0). The arrow denotes the position of elution of an added uv-absorbing marker 4-(p-nitrobenzyl)pyridine. "Reproduced with permission from Ref. 15. Copyright 1980, IRL Press".

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