Immunochemical studies on the

Chapters 17 through 21 deal with carbohydrate-enzyme systems. Hehre presents some new ideas on the action of amylases. Kabat presents some new immunochemical studies on the carbohydrate moiety of certain water-soluble blood-group substances and their precursor antigens. Hassid reviews the role of sugar phosphates in the biosynthesis of complex saccharides. Pazur and co-workers present information obtained by isotopic techniques on the nature of enzyme-substrate complexes in the hydrolysis of polysaccharides. Gabriel presents a common mechanism for the production of 6-deoxyhexoses. An intermediate nucleoside-5 -(6-deoxyhexose-4-ulose pyrophosphate) is formed in each of the syntheses. 

Immunochemical Studies on the Carbohydrate Moiety of Water Soluble Blood Group A, B, H, Lea, and Leb Substances and Their Precursor I Antigens... 

Immunochemical Studies on the Combining Sites of Forssman Hapten Reactive Hemagglutinins from Dolichos biflorus, Helix pomatia, and Wistaria floribunda, D. A. Baker, S. Sugii, E. A. Kabat, R. M. Ratcliffe, P. Hermentin, and R. U. Lemieux, Biochemistry, 22 (1983) 2741-2750. 

Wakizaka, A. and Okuhara, E. (1979) Immunochemical studies on the correlation between conformational changes of DNA caused by ultraviolet irradiation and manifestadon of andgeniciiy.y. Btochem. 86,1469-1478. 

Ram, D., Romano, B. and Schechter, I. (1994) Immunochemical studies on the cercarial-specific calcium binding protein of Schistosoma mansoni. Parasitology 108 289-300. 

Noshiro, M. and T. Omura (1978). Immunochemical study on the electron pathway from NADH to cytochrome P-450 of liver microsomes. 

Noshiro M, Harada N, Omura T (1980) Immunochemical study on the route of electron transfer from NADH to NADPH to cytochrome P-450 of liver microsomes. J Biochem 88 1521-1535 Nott JA, Moore MN (1987) Effects of polycyclic aromatic hydrocarbons on molluscan lysosomes and endoplasmic reticulum. Histochem J 19 357-368... 

Nemere, I. and CampheU, K. 2000. Immunochemical studies on the putative plasmalenunal receptor for 1, 25-dihydroxyvitamin D(3). m. Vitamin D status. Steroids 65(8) 451-5. 

Regier DA, Narrow WE, Rae DS The epidemiology of anxiety disorders the Epidemiologic Catchment Area (ECA) experience. J Psychiatr Res 24 3-14, 1990 Rehfeld JF Immunochemical studies on cholecystokinin, II distribution and molecular heterogeneity in the central nervous system and small intestine of man and hog. J Biol Chem 253 4022-4030, 1978... 

Layug, E., Immunochemical Studies on Blood Groups XXXIII. The Effects of Alkaline Borohydride and of Alkali on Blood Group A, B, and H substances, Biochemistry (1965) 4,1632. 

G. Schiffman, E. A. Kabat, and W. Thompson, Immunochemical studies on blood groups. XXXn. Immunochemical properties of and possible partial structures for the blood group A, B, and H antigenic determinants, Biochem. J., 3 (1964) 587-593. 

Immunochemical Studies on Blood Groups LXIX The Conformation of the Trisaccharide Determinant in the Combining Site of Anti-I Ma (Group 1), E. A. Kabat, J. Liao, M. H. Burzynska, T. C. Wong, H. Thogerson, and R. U. Lemieux, Molecular Immunol., 18 (1981) 873-881. 

The concept of receptors is credited to the independent work of Paul Ehrlich (1845-1915) and J. N. Langley (1852-1926). With Ehrlich, the concept appeared to originate from his immunochemical studies on antibody-antigen interactions. Based on the high degree of specificity of antibodies for antigens, Ehrlich postulated the existence of stereospecific, complementary sites on the two molecules. Similar... 

The original experiments on PDI activity were carried out with microsomal membranes, suggesting the localization of this protein in the endoplasmic reticulum. This has since been conhrmed both by subcellular localization studies (Lambert and Freedman, 1985) and by immunochemical methods (Koch, 1987). However, these studies revealed that PDI does not behave like a typical ER membrane protein. Indeed, for some time there was a question as to whether there was more than one form of PDI due to the presence of this protein in the cytosol. This has now been discounted by studies on the latency of PDI that show that if microsomes are prepared carefully then the activity is entirely latent (Lambert and Freedman, 1985). Thus, although PDI exists as an ER protein, it is easily released from microsomes by freeze thawing, mild alkaline treatment, detergents, or sonication, suggesting that PDI is either a soluble protein or is only loosely associated with the ER membrane. 

Kla. Kabat, E., Bendich, A., and Bezer, A. E., Immunochemical studies on blood groups. II. Properties of the blood group A substance from pools of hog stomach of specific precipitates composed of A substance and homologous human antihody. J. Exptl. Med. 83, 447 (1946). 

R12. Reichert, L. E., Kathan, R. H., and Ryan, R. J., Studies on the composition and properties of immunochemical grade human pituitary follicle-stimulating hormone (FSH) Comparison with luteinizing hormone (LH). Endocrinology 82, 109-114 (1968). 

H13. Heremans, J. F., Immunochemical studies on protein pathology. The immunoglobulin concept. Clin. Chim. Acta 4, 639-646 (1959). 

Kabat EA, Turino GM, Tarrow AB, Maurer PH. Studies on the immunochemical basis of allergic reactions to dextran in man. J Clin Invest 1957 37 1160-1170. 

Loor F, Roelants GE (1977) B and T cells in immune recognition. Wiley, New York Mage RG, Kabat EA (1963) Immunochemical studies on dextran. III. The specificities of rabbit antidextrans. Further findings on antidextrans with 1,2- and 1,6-specificities. J Immunol 91 633-640... 

Schlossman SF, Levine H (1967) Immunochemical studies on delayed and arthus-type hypersensitivity reactions. I. The relationship between antigenic determinant size and antibody combining site size. J Immunol 98 211-219 Schlossman SF, Yaron A, Ben-Efraim S, Sober HA (1965) Immunogenicity of a series of, N-DNP-L-lysines. Biochemistry 4 1638-1645 Schlossmann SF, Levine H, Yarou A (1968) Studies on the specificity of antibody to 2,4-dinitrophenyl-poly-L-lysines. Biochemistry 7 1-7 Schneider CH, de Week AL (1967) The reaction of benzylpenicillin with carbohydrates at neutral pH with a note on the immunogenicity of hapten polysaccharide conjugates. Im-munochemistry 4 331-343... 

In order to identify the amino acid residues in the antigenic reactive sites, the following three approaches have so far been pursued in BSA (1) studies on the immunochemistry and conformation of chemical derivatives of BSA specifically modified at particular amino acid residues, (2) the isolation of immunochemically reactive fragments liberated by proteolytic digestion of the protein, and (3) studies of the effects of chemical modification of selected amino acid residues on the immunochemistry and conformation of these immunochemically reactive peptides. 

Serum albumin, the most abundant plasma protein, has been subjected to extensive studies which resulted in a wealth of information on its chemical and physicochemical properties. Only recently has the amino acid sequence of bovine and human serum albumins become known. Prior to the availability of the amino acid sequence, immunochemical studies on BSA or HSA were hampered by the inability to assign an immunochemically reactive fragment within the sequence of the albumin molecule. With the availability of the sequence, it is becoming possible to identify immunochemically reactive fragments of known sequences. Thus fragments 11-193 and 377-571 were isolated from BSA. These fragments were shown to have rather unusual immunochemical properties. Each... 

Each monomer contains one pyridoxal phosphate and a highly conserved primary structure in the vicinity of the cofactor binding site. The enzymes follow the same catalytic mechanism, a rapid equilibrium random Bi Bi mechanism. However, unlike animal phosphorylases, the microbial and plant enzymes are not subjected to covalent or allosteric control. Within the group of higher plant phosphorylases two types of enzyme have been distinguished which differ in monomer size, peptide pattern, glucan specificity, and intracellular location (1-3). Based on immunochemical studies on leaf tissues (4-5), one enzyme form has been localized in the cytosol whereas the other one resides in the chloroplast. Thus, the two plant phosphorylase types represent non-interconvertible proteins which presumably have entirely different metabolic functions. 

M.Torii, E. a. Rabat and H. Weigel, Immunochemical Studies on Dextrans. IV. Further Characterization of the Determinant Groups on Various Dextrans Involved in Their Reactions with the Homologous Human Antidextrans, J. Immun. 96, 797-805 (1966). 

Immunochemical studies on galactomannans isolated from the mycelia and culture media of Hormodendrum pedrosoi, H. compactum, and H. dermatitidis have indicated that acid-labile D-galactofuranosyl residues are responsible for precipitating antibodies. The galactomannans and their respective core components precipitated anti-(5. cerevisiae) serum, which interacts only with (1 6)-... 

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