Dolichos biflorus lectin isolation

A lectin, isolated from the seeds of Amphicarpaea bracteata by affinity chromatography on 2-acetamido-2-deoxy-D-galactose coupled to epoxy-activated agarose, has been shown to have characteristics common with Dolichos biflorus lectin, especially in relation to the agglutination of human Ai erythrocytes. It is assumed that the lectin interacts with non-reducing terminal 2-acetamido-2-deoxy-D-galactosyl residues on the red cell membrane of these erythrocytes. 

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