DNA genomics

The most widely used DNA vims vectors are derived from adenovirus (Ad) and adeno-associated viius (AAV). Adenoviruses contain a 36-kb double-stranded DNA genome and can infect a broad spectrum of cells, including nondividing cells like hepatocytes and neuronal cells. Ad vectors can be produced at high... 

Approaches to cloning DNA Genomic DNA Restriction endonucleases fragment DNA Total nuclear DNA cloned Genes contain introns cDNA Reverse transcription of mRNAs from cell Genes expressed cloned Genes have no introns... 

The Baculoviridae are a family of large enveloped DNA viruses that are characterised by rod-shaped nucleocapsids and relatively large double stranded DNA genomes. Autographa californica Multicapsid Nuclear Polyhedrosis Virus (AcMNPV) is the baculovirus most currently used as vector for protein production with insect cells. Several reviews are available describing baculovirus structure and its molecular biology [6-8]. 

DNA genome, translated on RNA-protein complex (ribosome) with protein catalysts... 

The T-odd bacteriophages Tl, T3, T5, and T7 are medium-sized phage with linear duplex DNA genomes. Replication of linear DNA in these and in many other genomes presents a problem. Even if the RNA primer segment is made at the very 3 end of the template strand, there will be a gap in the final replicated strand when the primer is digested out. Since there is no known enzyme that will add to the 3 end of a chain, this gap will remain unfilled. The problem is solved by terminal redundancy, the presence of a common 260-nucleotide... 

You are given a sample of nucleic acid extracted from a virus. How would you determine whether the virus has an RNA or DNA genome and whether it is single- or double-stranded ... 

Hepatitis B is an animal virus with a small circular DNA genome. A gap in one strand is bridged by an incomplete complementary strand. The longer strand has a protein bound at its 5 end. A DNA polymerase present in the mature virus particle can elongate the 3 ends of the incomplete strands. An unusual feature of this virus is that replication involves an RNA intermediate that must be reverse transcribed. 

Single stranded RNA molecules are synthesized by a template mechanism from select regions of the DNA genome. 

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