Genomic DNA extraction

DNA amplification Genomic DNA, extracted from nuclei or mitochondria, may be amplified by a polymerase chain reaction (PCR). 

An initial reverse in situ hybridization is carried out using genomic DNA extracted from the test cell lines and compared to normal DNA controls. A visual... 

Handling and Storage of Samples for Genomic DNA Extraction DNA is a relatively stable molecule. However, some precautions are recommended for handling and storage of specimen containing DNA or isolated DNA samples. 

Figure 1 Replica dot-blots of genomic DNA extracted from four adult female S. soubrense B (row A), yahense (Row B) and S si rbanum (row C), arTd probed with pS03, pSOll and pSQl.

SAMNP-mediated genomic DNA extraction is relatively simple (free from filtration and centrifugation), fast (30 min), and environmental friendly (free of toxic chemicals). It can be used to extract genomic DNA from crude cell culture media without shearing, and is ideal for use in genomic sequencing applications. 

Figure 44.2 Analysis of 5-methyl cytosine (% 5-metC) and dUTP/dTTP (dU/dT) ratio in genomic DNA of HepG2 cell line. Folate depletion results in decrease of 5-metC% and elevation of dU/dT into genomic DNA extracted from HepG2 cell lines grown in complete medium (ECM) or in folate depleted medium (FDM) for 72h (T72) control (TO). (Chango et al. 2009a).

Genomic-DNA Extraction and Southern-Blot Analysis for Recombinants (standard reagents for molecular biology are not noted)... 

Passmore, L. J. Killeen, A. A. Toluidine blue dyebinding method for measurement of genomic DNA extracted from peripheral blood leukocytes. Mol. Diagn. 1996, 1, 329-334. 

Genomic DNA extraction kits (fcc Note 4) For 1-96 samples use individual genomic extraction kits. For >96 samples, 96-well genomic extraction kits are preferred. 

Harvest the cells after 48 h of growth by centrifugation and remove the supernatant, saving the pellet. Proceed to genomic DNA extraction (Subheading 3.5) or store a cell pellet at -80 °C until extraction. Make sure to remove all cells from the wells by pipetting up and down. 

Individual cultures Perform genomic DNA extractions on the 200 pL cultures, scaling the kit specifications to the smaller volume. We have found that eluting the DNA with 35-50 pL of elution buffer gives a good concentration for PCR. 

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