Hepatocyte-like cells

Gutierrez, E., Wiggins, D., Fielding, B. and Gould, A. P. (2007). Specialized hepatocyte-like cells regulate Drosophila lipid metabolism. Nature, 445, 275-280. 

Hai DC. 2007. Direct differentiation of human embryonic stem cells to hepatocyte-like cells exhibiting functional activities. Cloning Stem Cells 9, 51-62. 

Soderdhal T. 2007. Glutathione transferases in hepatocyte-like cells derived from human embryonic stem cells. 

Soto-Gutierrez A. 2007. Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Nat. Protoc. 2, 347-356. 

Manipulation of cell environment, differentiation, and stability Production of desired cells at will Still much work needed to identify crucial factors Stem cell-derived hepatocyte-like cells... 

Jozefczuk J, Prigione A, Chavez L, Adjaye J (2011) Comparative analysis of human embryonic stem cell and induced pluripotent stem cell-derived hepatocyte-like cells reveals current drawbacks and possible strategies for improved differentiation. Stem Cells Dev 20 1259-1275... 

Rambhatla L, Chiu CP, Kundu P, Peng Y, Carpenter MK. Generation of hepatocyte-like cells from human embryonic stem cells. Cell Transplant. 2003 12(1) 1-11. 

Yamada T, Yoshikawa M, Kanda S, et al. (2002). In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. Stem Cells. 20 146-154. 

Endoderm. MAPCs can differentiate into hepatocyte-like cells by using FGF4 and HGF. Such cells expressed hepatic markers of CK19, AFP, CK18, albumin, HepPar-1, and CD26 and produced albumin, urea, and glycogen [30]. 

Schwartz R E, Reyes M, Koodie L, et al. (2002). Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J. Clin. Invest. 109 1291-1302. 

Lardon J, De Breuck S, Rooman I, et al. (2004). Plasticity in the adult rat pancreas transdifferentiation of exocrine to hepatocyte-like cells in primary culture. Hepatol. 39 1499-1507. 

Plainly DILI is a major cause for concern toward which efforts to detect potential hepatotoxicants before they reach later stages of development should be made. During development, molecules must be tested using in vitro models before progressing to animal and human trials. There are several in vitro models available including primary human hepatocytes, inunortalized cell lines and animal models, and, the main subject of this chapter, pluripotent stem cell-derived hepatocyte-like cells (HLCs). 

Chen, Y. R, Tseng, C. Y, Wang, H. W., Kuo, H. C., Yang, V. W., Lee, O. K. 2012. Rapid generation of mature hepatocyte-like cells from human induced pluripotent stem cells by an efficient three-step protocol. Hepatology, 55, 1193-203. 

Cerec, V., et al., Transdifferentiation of hepatocyte-like cells from the human hepatoma HepaRG cell line through hipotent progenitor. Hepatology, 2007. 45(4) p. 957-67. 

Wilson s disease Defective copper transport in iPSC-derived hepatocyte-like cells [102]... 

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