Eukaryotes genomic DNA

Sambrook J, Fritsch EF, Maniatis T (1989) Analysis and cloning of eukaryotic genomic DNA. In Molecular Cloning A Laboratory Manual, 2nd ed., N Nolan, C Nolan, and M. Ferguson, Eds., pp 9.31-9.62. Cold Spring Harbor Laboratory Press, Plain-view, NY. 

Eukaryotic genomic DNA consists of three major classes of sequences genes encoding proteins and functional RNAs, including gene families and tandemly repeated genes repetitious DNA and spacer DNA (see Table 10-1). 

In eukaryotic genomes, DNA transcription control elements may be located many kilobases away from the promoter they regulate. Different control regions can control transcription of the same gene in different cell types. 

Eukaryotic genomic DNA does not display much variation between species. The %(G -i- C) composition of most plant and animal species falls within a narrow range, averaging 39% with a variation of only +6%. The... 

Kagiya, G. et al (2005) Generation of a strong promoter for Escherichia coli from eukaryotic genome DNA. /. Biotechnol, 115 (3), 239 -248. 

The protein coding regions of DNA, the transcripts of which ultimately appear in the cytoplasm as single mRNA molecules, are usually interrupted in the eukaryotic genome by large intervening sequences of... 

The DNA in a eukaryotic genome can be divided into different sequence classes. These are unique-sequence, or nonrepetitive, DNA and repetitive-sequence DNA. In the haploid genome, unique-sequence DNA generally includes the single copy genes that code for proteins. The repetitive DNA in the haploid genome includes sequences that vary in copy number from two to as many as 10 copies per cell. 

In the nuclei of all eukaryotic cells, DNA is tightly wrapped around an octamer of histone proteins and is compacted into a dense structure known as chromatin. In order to access the genetic information which is required in numerous essential cellular processes including DNA replication, gene expression and DNA repair, chromatin needs to be partially unwound. One important mechanism to regulate chromatin structure and thus to control the access of the genomic DNA is through histone modifications [1-6]. The histone octamer is composed of two copies of H2A, H2B, H3 and H4 core histone proteins. Their tails, that protrude out of the surface of the... 

In eukaryotes, conservation of the exon order of orthologous genes among species is an invaluable tool for their prediction. Exons can be predicted from genomic DNA by alignment with proteins (Gelfand et al., 1996) or with cDNAs (Florea et al., 1998), which in principle do not contain introns. However, a new, more radical approach is under development. In an alignment at the DNA level, exons can be identified... 

Abstract The DNA in eukaryotic genome is compartmentalized into various domains by a... 

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