Integration of T-DNA into the Host Genome

Little is known about T-DNA integration into the genome of the host cell integration appears to take place in a random and nonsequence-spe-cific manner. The number of T-DNAs and the manner in which they are inserted can be complex. For example, it is not uncommon for two T-DNA molecules to be found inserted in a head-to-head orientation within a plant genome. 

It is currently believed that double-stranded break (DSB) repair enzymes may act as baits to attract invading T-DNA molecules to the sites of integration. This DSB repair machinery may be transported via histone modifications as part of a general process of intranuclear protein traffic that also allows transcriptional factors to reach their target promoters (Lacroix et al., 2006b). 

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