Dissecting microscope

The proglottid is examined under a dissecting microscope or with a hand lens, and the uterine branching is observed. Glycerine and beechwood creosote can also be used with good results. Cleared proglottids may be mounted or stained if desired. 

H5. Holmes, R., Hourihane, D. O., and Booth, C. C., Dissecting-microscope appearances of jejunal biopsy specimens from patients with idiopathic steatorrhoea. Lancet i, 81-83 (1961). 

For ligand-gated ion channel receptor expression studies, stage V and VI oocytes are preferred (see earlier). Under a dissecting microscope, oocytes of this stage that are in good condition are sorted from the rest and then incubated at 16-18°C in MBM overnight. Incubation in hypertonic phosphate... 

Look at the crystals carefully imder a dissecting microscope equipped with polarizers. Often crystals... 

For each treatment arm, the values represent the means the standard deviation of the number of polyps per whole mouse intestine, as observed under a dissecting microscope. In each case, mice were fed the indicated diets for 60 d after weaning, then killed for determination of polyp number. Each group consisted of 14-15 mice. 

The maternal ovaries are removed and examined at the time of necropsy and corpora lutea are counted. The corpus luteum (CL) is a transitory endocrine organ formed from the thecal and granulosa cells of the postovulatory follicle... Information on the number of copora lutea is useful in the interpretation of data on the viability of conceptuses (see below, pre-implantation loss). Corpora lutea counts can be conducted with fresh or fixed tissue, and are generally performed with the aid of a dissecting microscope. Minimal dissection of the ovarian tissue is required (13). 

Due to the small size of the mouse, fetal examinations should be performed using a stereo dissecting microscope. 

Stereo dissecting microscope with lx objective and lOx eyepieces (see Note 2). 

Check the staining status occasionally under a dissection microscope since the staining can show up quickly, but minimize exposure of samples to light. Generally, the color develops within 20 min at room temperature if the gene expression level is relatively high. 

Tumor colonies that differ in color from the lung parenchyma (melanoma) can be counted with the aid of a dissecting microscope. 

Most pulmonary tumor colonies in mice are in the pleura. Therefore, pigmented colonies (such as melanomas) can be counted without lung dissection. However, it may be more facile, especially when there are extensive pulmonary metastases, to dissect the lungs into the individual four or five lobes which are then enumerated individually using a dissecting microscope. 

The lungs are rinsed in water to remove excess Bouin s solution, and the tumor colonies counted with the aid of a dissecting microscope (see Note 9). Murine lungs with greater than 300 colonies should be reported as >300 or too numerous to count. 

Radish seeds were treated with RO fraction at dilutions 1 8, 1 10 and 1 14 as described above and were processed for electron microscopy 46 h after the start of the treatment. Roots from control seeds, moistened with distilled water, were fixed after 2, 16 and 46 h in order to get several developmental stages as references for treated seeds. The root apex, about 1 mm long, was cut under a dissecting microscope from at least 10 seeds and fixed in the primary fixative for 2 h at room temperature under mild vacuum. Since the control seeds had not yet started germination after 2 h imbibition and nearly 100% of the seeds treated with 1 8-diluted had not germinated after 46 h, in these two cases the seed integument was cut open with a razor blade in order to dissect the root. 

Transfer 1 oviduct at a time to another Petri dish containing M2 supplemented with 300 ug/ml. hyaluronidase and place under a dissecting microscope. Using forceps, tease the oviduct open to release eggs. Allow the hyaluronidase digestion to remove the cumulus cells, but as soon as they are gone, transfer the egg to a fresh dish of M2 medium without hyaluronidase. 

Locate the infundibulum under a dissecting microscope, and create a hole in the bursa with fine forceps. Pick up the infundibulum and insert the transfer pipet into the ampulla. Using a mouth pipet, expel the two bubbles flanking the eggs into the uterus. 

Dissection microscope with needles and appropriate manipulation devices for blastocysts. 

Using a dark-field dissecting microscope, aspirate one colony into the capillary tube by mouth suction and transfer it into one of the wells of a 96-well U-bottom plate. Collect 16 colonies (i.e., 2 vertical rows) every time. 

Fig. 1 Comparisons of the excess viscosity measurements y (%) obtained from bulk phase seawater samples after removing all the visible colonies from the water samples with a Pasteur pipette under a dissecting microscope (magnification x 100), i Pasteur> and after carefully screening the water samples through a 200 pm mesh (J72oo/im) before doing the measurements. A modified t-test (Zar 1996) did not exhibit a significant difference between our measurements and the theoretical expectation J7Pasteur = y2Q0flm (P > 0.05, N = 100)...

We can readily extend our discussion to include a pressure-volume, or P-K curve, which has proved useful for analyzing the water relations of plant organs such as leaves. In particular, the earliest calculations of internal hydrostatic pressure and cellular elastic modulus were based on P-V curves. To obtain such a curve, we can place an excised leaf in a pressure chamber (Fig. 2-10) and increase the air pressure in the chamber until liquid just becomes visible at the cut end of the xylem, which is viewed with a dissecting microscope or a hand-held magnifying lens so that water in individual conducting cells in the xylem can be observed. When the leaf is excised, the... 

Alternatively, wells can be photographed by transmitted light under a dissecting microscope. Total areas of cell outgrowth of both time zero control and test wells are digitized using a Sigma-Scan... 

But when flowers or other plant parts are to be dissected, it is necessary to have both hands free. To meet this need various forms of stands have been devised which have been combined with an arm and lens to constitute what are known as Dissecting Microscopes. One of the simpler forms of these is shown in Figs. I and 2. It consists of a low wooden stand with inclined sides that furnish convenient hand rests for the operator. In the center of... 

Pig, I.—Front view of a dissecting microscope. Description in text. 

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