Pipetting mouth

Prohibit eating, drinking, and all mouth-pipetting in rooms containing designated radioactivity areas. 

In industrial use, some instances of skin and respiratory tract irritation have been observed but no chronic effects have been reported. A human exposure to 12,000 ppm for 1.5 minutes in a laboratory produced nose irritation and cough mouth pipetting of the liquid caused a severe sore throat and reddened mucous membranes. Workers exposed for several hours to low vapor concentrations complained of foggy vision with rings around lights, the results of corneal edema, which cleared within 3-4 hours after cessation of exposure. ... 

Acrylamide in the unpolymerized form is a skin irritant and a potential neurotoxin. Wear gloves and a mask while weighing the dry powder. Do not breathe the dust. Prepare all acrylamide solutions in the hood. Do not mouth pipet any solutions used for gel formation or staining. 

Ethidium bromide is a mutagen and a potential carcinogen. Always wear gloves and a face mask while weighing or handling the pure chemical. Gloves should always be worn while using solutions of the dye Do not allow the solutions of ethidium bromide to come into contact with your skin. Do not mouth pipet any ethidium bromide solutions. Save all assay mixtures and excess ethidium bromide solutions after measurements are taken, and pour them into a container marked Waste Ethidium. ... 

Locate the infundibulum under a dissecting microscope, and create a hole in the bursa with fine forceps. Pick up the infundibulum and insert the transfer pipet into the ampulla. Using a mouth pipet, expel the two bubbles flanking the eggs into the uterus. 

Lood should be stored in cabinets or refrigerators designated for such use only. Mechanical pipetting devices should be used and mouth pipetting prohibited. All hazardous chemicals should be used in a chemical fume hood. 

Mouth pipeting is prohibited mechanical pipeting devices are used. 

Safety pipetting aids Mouth pipeting is prohibited it is a potential source of infection either by ingestion or by inhalation. All safety pipetting aids control contamination of the suction end of the pipet and leakage from the pipet tips. Vacuum lines used to aspirate liquids are protected with liquid disinfectant traps and cartridge-type filters (0.45 pm pore size). 

Never perform mouth pipetting and never blow out pipettes that contain potentially infectious material. 

Strong acids, caustic materials, and strong oxidizing agents should be dispensed by a commercially available automatic dispensing device. Under no circumstances is mouth pipetting permitted. 

No mouth pipetting - ose automatic pipettes or pipetting devices. 

Mouth pipetting is prohibited mechanical pipetting devices are to be used. 

Mechanical or automatic pipetting devices must be used. Mouth pipetting is prohibited. 

Transfer embryos from the culture to the manipulation drop in the petri dish by mouth pipeting. 

For embryos operated in New culture (J3, 4] Chapter 18), the glass ring should first be flooded with CMF, and the edges of the area opaca detached from the vitelline membrane. Then, pick up the embryo with a wide-mouthed pipet or with fine forceps (to grasp the extraembryonic membranes) and transfer it to a Sylgard dish for pinning and fixing as just described. 

With Millipore forceps, gently squeeze this area while collecting sperm in a glass capillary pipet attached by thin-gage mbing to a mouth pipet or other suction device. 

Mouth pipetting in the laboratory can lead to the ingestion of toxic chemicals. But the more common cause of unintentional ingestion is from foods that were stored in containers that had previously been used to store nonfood items (paint, plant food, or other substances). Another common, but very unsafe, practice that can lead to ingestion is storing food in a place where chemicals are stored—or storing chemicals in a refrigerator used for food. 

Do not put anything in your mouth under any circumstances while in the laboratory. This includes food, drinks, chemicals, and pipets. There are countless ways that surfaces can become contaminated in the laboratory. Since there are substances that must never be pipetted by mouth, one must get into the habit of never mouth pipetting anything. 

All procedures involving blood or other potentially infectious materials must be performed in such a manner as to minimize spattering, generating droplets, splashing, and spraying. Mouth pipetting/suctioning of blood or other potentially infectious materials is prohibited. 

One technique that should be avoided is the practice of mouth pipetting. Although common among earlier generations of microbiologists, this practice should be prohibited due to safety concerns. In place, a number of relatively inexpensive pipetting devices are now available. 

Never taste any laboratory chemical. Mouth-pipetting is prohibited in laboratories. 

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