Disaccharides enzymic hydrolysis

Oligosaccharide derivatives of uridine 5 -pyrophosphate are probably present in higher plants. Cellobiose and a disaccharide composed of a glucose and an arabinose were identified among products of enzymic hydrolysis of a uridine diphosphate sugar fraction from larch wood.20... 

Invert sugar. Invert sugar, sometimes referred to as partially inverted refiner s syrup, is produced by acid or enzymic hydrolysis of the disaccharide sucrose into its 2004 component pails of fructose and dextrose (glucose). Invert syrups usually contain a mixture of sucrose, fructose and dextrose. The main advantage of such a syrup is the reduced likelihood of crystallisation and an increase in osmolality, which may be useful in reducing spoilage risk. 

A drawback in the synthesis of disaccharides by transglycosidation is the enzymic hydrolysis of the product by the glycosidase. In Section 10.4.3, we saw... 

Sucrose is the sole source for synthesis of D-fructan in artichoke tubers.206 Thus, the level of this disaccharide can play an important role in the regulation of enzyme activities.202,203,207 Sucrose strongly inhibits the enzymic hydrolysis of inulin by D-fructan hydrolases, as... 

The insoluble polysaccharide was characterized by examination of the soluble, " G-labeled oligosaccharides isolated by a variety of treatments, including enzymic hydrolysis, partial hydrolysis with acid, or acetolysis. From the polymer containing D-mannose- C, several oligosaccharides were isolated having various ratios of D-mannose to D-glucose, from 1 1 to 3 or 4 1. One of these oligosaccharides was a disaccharide tentatively identified as an 0-/3-D-man-... 

The h.p.l.c. analysis of unsaturated disaccharides obtained by enzymic hydrolysis of heparan sulphate and heparin was found to be more rapid and much more sensitive than the previously described method which employed paper chromatography. ... 

Active immobilized enzyme hydrolysis of synthetic disaccharides... 

Rate of Enzymic Hydrolysis op Disaccharides and Related Compounds... 

Disaccharides from glycosanunoglycans. - Unsaturated disaccharides released by enzymic hydrolysis of chondroitin sulphate have been separated on a primary amino-containing gel column, those from hyaluronic acid, chondroitin sulphate and dermatan sulphate by reversed-phase h.p.l.c. on an amido-phase as their dansyl hydrazone derivatives with chemiluminescence detection," and those from dermatan sulphate and heparin by ion-pair reversed-phase microbore h.p.Lc. coupled with ion-spray m.s. for use in monitoring their presence in patients treated vrith these polysaccharides intravenously. ... 

The structures and mole % of the total xyloglucan accounted for by each of these peaks are summarized in Fig. 6. The location of the Fuc-(1 2)-Gal disaccharide in the nonasaccharide was not originally determined (57). It has since been determined by partial enzymic hydrolysis of the nonasaccharide and isolation of the resulting definitive pentasaccharide labeled d in Fig. 6 (729). 

For the most part, low molecular weight carbohydrates of commerce are made by depolymerization via enzyme- or acid catalyzed hydrolysis of polysaccharides. Only sucrose and, to a very much lesser extent, lactose, both disaccharides, are commercial low molecular weight carbohydrates not made in this way. 

The glycosidic bond to an anomeric carbon can be either a or (3. Maltose, the disaccharide obtained by enzyme-catalyzed hydrolysis of starch, consists of two cv-D-glucopyranose units joined by a 1->4-o-glycoside bond. Cellobiose, the disaccharide obtained by partial hydrolysis of cellulose, consists of two /3-o-glucopyranose units joined by a 1—>4-/3-glycoside bond. 

---