Oligosaccharides labeling

Functional drinks continue to be very successful in Japan and the major ingredients exploited there are fibre, calcium, iron and oligosaccharides. Label text refers to beneficial effects on the digestive system, bones, teeth and other parts of the body as appropriate. In Europe these drinks are only beginning to be developed. Examples that are available in the UK include ... 

Guttman, A., Chen, F.A., Evangelista, R.A. and Cooke, N. (1996) High-resolution capillary gel electrophoresis of reducing oligosaccharides labeled with l-aminopyrene-3,6,8-trisulfonate. 

Cazzulo, J. J., and Parodi, A. J. (1982). Protein glycosylation in Trypanosoma cruzi. II. Partial characterization of protein-bound oligosaccharides labeled in vivo. J. Biol. Chem. 257, 7641-7645. 

Lucas et al (1975) showed that the transfer of the oligosaccharide to protein took place en bloc. They prepared large dolichyl pyrophosphoryl oligosaccharides, labelled with [ " Cjmannose and A/-[ H]acetylglucosamine, and showed that both labels were transferred from them to endogenous... 

This support, which is of widespread use in the field of protein and DNA electrophoresis, is applied for the separation of oligosaccharides, labeled by fluoro-phores like 2-aminoacridone or 8-aminonaphthalene-1,3,6-trisulfonate, formed by enzymatic hydrolysis of glycoproteins. For this purpose small-pore gel material is used either with uniform pores or as... 

Franz, A.H. MoUnski, T.F. Lebrilla, C.B. MALDI-FTMS Characterization of oligosaccharides labelled with 9-amino-fluorene. J. Am. Soc. Mass Spectrom. 2001,12,1254-1261. 

The smallest oligosaccharide labelled in the mannose was formed by incubation of GDP-Man with Dol-P-P-di N,acetylchitobiose and is presumably the trisaccharide mannosyl diacetylchitobiose (Levy et al., 1974). It should be noted that in many oligosaccharides the mannose joined to the diacetylchitobiose is 3 whereas the mannoses in the chains attached to it in the 3 and 6 position are all a. There is evidence suggesting that these two different types of bonds may arise through transfer reactions involving different sugar donors. In the first case it may be GDP-Man which has an a configuration and in the latter Dol-P-Man which is 3. ... 

Wuhrer, M. Deelder, A. M. Negative-mode MALDI-TOF/TOF-MS of oligosaccharides labeled with 2-aminobenzamide. Anal. Chem. 2005, 77, 6954-6959. 

Min, J. Z. Kurihara, T Hirata, A. Toyo oka, T. Inagaki, S. Identification of A-linked oligosaccharide labeled with 1-pyrenesulfonyl chloride by quadrupole time-of-flight tandem mass spectrometry after separation by micro- and nanoflow liquid chromatography. Biomed. Chromatogr. 2009, 23, 912-921. 

Kotani, N. and Takasaki, S., Analysis of 2-aminobenzamide-labeled oligosaccharides by high-pH anion-exchange chromatography with fluorometric detection, Anal. Biochem., 264, 66, 1998. 

Figure 21 Separation of ANTS-labeled oligosaccharides. (From Guttman, A., Cooke, N., and Star, C. M., Electrophoresis, 15, 1518, 1994. With permission.)...

Biotinylated oligosaccharides are convenient probes of carbohydrate interactions, because the biotin label can be captured or detected using an avidin or streptavidin derivative. For instance, immobilized streptavidin can be used to purify glycoconjugates that have been labeled... 

O Shannessy, D.J., Doberson, M.J., and Quarles, R.H. (1984) A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties. Immunol. Lett. 8, 273-277. 

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