Dermatan sulphate

Scully, M. F., Ellis, V., Seno, N., and Kakkar, V. V. (1988). Effect of oversulphated chondroitin and dermatan sulphate upon thrombin and factor Xa inactivation by antithrombin III or heparin cofactor II. Biochem. J. 254,547-551. 

Dermatan sulphate (condroitin sulphate B from pig skin). Purified by digestion with papain and hyaluronidase, and fractionation using aqueous EtOH. [Gifonelli and Roden Biochemical Prepreparations 12 1 1968]. 

Heparin (from pig intestinal mucosa). Most likely contaminants are mucopolysaccharides including heparin sulphate and dermatan sulphate. Purified by pptn with cetylpyridinium chloride from saturated solutions of high ionic strength. [Cifonelli and Roden Biochemical Preparations 12 12 1968]. 

The final Klebsiella polysaccharide that will be mentioned specifically is KZS, whose chemical structure was determined by Niemann et al (24) (Figure 30). This polysaccharide is of particular interesT because its backbone consists of a similar alternating 1, 3 diequatorial, 1, 4 diequatorial glycosidic linkage geometry to that found in the connective tissue polysaccharides, hyaluronic acid, chondroitin sulphate and dermatan sulphate (25). All these three polysaccharides have exhibited 3-fold helical conformations with axially projected chemical repeats in the range 0.95 - 0.97 nm which is comparable to 0.97 nm found in K25 which also forms a 3-fold helix (14). Further, left-handed helices were found to be more favourable in K25 as has previously been observed in the connective tissue polysaccharides. The similarities between these various different structures is apparent in Figure 31 which shows projections down the axis of K25 and several of the connective tissue polysaccharides. 

Chemical Name Suleparoid sodium salt (ap. 84%), mixture with dermatan sulphate sodium (ap. 12%) and chondroitin sulphate sodium (ap. 4%)... 

L. Fraosson and L Roddn. Structure of dermatan sulphate I. Degradation by testicular hyaluronidase. J. Bid Chem. 242 4161 (1967). 

M. Lyon and C. F. Phelps. A rapid purification of bovine testicular hyaluronidase by chromatography on dermatan sulphate-substituted 1,6-dlaminohexane-Scpharose 4B. 

Poulsen, J.H. and Cramers, M.K., Determination of hyaluronic acid, dermatan sulphate, heparan sulphate and chondroitin 4/6 sulphate in human dermis, and a material of reference, Scand. J. Clin. Lab. Invest., 42, 545, 1982. 

HS, heparin sulphate DS, dermatan sulphate KS, keratan sulphate HA, hyaluronic acid PC, phosphatidylcholine PL, phospholipid SL, sphingolipid. 

Lane DA, Ryan K, Irdand H, Curtis JR, Nurmohamed MT, Kredi RT, Roggdcamp MC. Stevens P, ten Cate JW Dermatan sulphate in haemodialysis. lancet (Ithrombocytopoiia and thrombosis. Australian Nen> Zealand JMed mi) 21(l) 52-54. 

Cofrancesco E, Boschetti C, Leonardi P, Cortellaro M Dermatan sulphate in acute leukaemia Lancet (1992) 339(8802) 1177-1178. 

Stoffyn, P. J., and Jeanloz, R. W., The identification of the uronic acid component of dermatan sulphate (fi-heparin, chondroitin sulfate B). J. Biol. Chem. 235, 2507-2510 (1960). 

The main constituents of the extracellular matrix are (1.) collagen, particularly the fibrillary types I (36%), III (36%) and V (16%) as well as the non-fibrillary types FV (9%), VI (0.2%) and to a minor degree also type VII, (2.) glycoproteins (fibronectin, tenascin, laminin, undulin, elastin, entactin), and (2.) proteoglycans of both the core protein and the glycosaminoglycan type (e.g. chondroitin, heparan sulphate, hyaluronic add, dermatan sulphate). 

V. Sinninger, J. Tapon-Bretaudiere, F. L. Zhou, A. Bros, et al.. Immobilization of dermatan sulphate on a silica matrix and its possible use as an affinity chromatogra-... 

We have also achieved suppression of scarring in the CNS using decorin, a small dermatan sulphate proteoglycan, which is a more universal antagonist of the TGF-P... 

B. Casu, D. R. Ferro, M. Ragazzi, and G. Torri, in J. E. Scott (Ed.), Dermatan Sulphate Proteoglycans. Chemistry, Biology, Chemical Pathology. Portland Press, London, 1993, pp. 41-53. 

Mucopolysaccharidoses This group of disorders is characterized by tissue accumulation of glycosaminoglycans such as heparin sulphate and dermatan sulphate. This results in skeletal deformities, mental retardation and premature death... 

Chondroitin sulphate B (/ -heparin, dermotoidin sulphate) Dermatan sulphate Al-acetylgalactos-amine-4-sulphate iduronic acid Aorta, heart valves, lig. nuchae, skin, tendon... 

Heparin monosulphuric acid Mixture of heparan sulphate, dermatan sulphate and heparin Intestine, lung... 

Chen YL, Lee HP, Chan HY et al (2007) Composite chondroitin-6-sulfate/dermatan sulphate/ chitosan scaffolds for cartilage tissue engineering. Biomaterials 28 2294—2305... 

L.-A. Fransson, Periodate oxidation of L-iduronic acid residues in dermatan sulphate, Carbohydr. Res., 36 (1974) 339-348. 

Chopra RK, Pearson CH, Pringle GA, Fackre DS, Scott PG. Dermatan sulphate is located on serine-4 of bovine skin proteodermatan sulphate. Demonstration that most molecules possess only one glycosaminoglycan chain and comparison of amino acid sequences around glycosylation sites in different proteoglycans. Biochem J 1985 232 277-279. 

Cheng F, Heinegard D, Malmstrom A, Schmidtchen A, Yoshida K, Fransson LA. Patterns of uronosyl epimerization and 4-/6-0-sulphation in chondroitin/ dermatan sulphate from decorin and biglycan of various bovine tissues. Gly-cobiology 1994 4 685-696. 

Scott PG, Dodd CM. The A-linked oligosaccharides of bovine skin proteo-dermatan sulphate. Biochem Soc Trans 1989 17 1031-1032. 

Fransson LA. Interaction between dermatan sulphate chains. I. Affinity chromatography of copolymeric galactosaminioglycans on dermatan sulphate-substituted agarose. Biochim Biophys Acta 1976 437 106-115. 

Kuwaba K, Kobayashi M, Nomura Y, Irie S, Koyama Y. Elongated dermatan sulphate in post-inflammatory healing skin distributes among collagen fibrils separated by enlarged interfibriUar gaps. Biochem J 2001 358 157-163. 

Fushimi H, Kameyama M, Shinkai H. Deficiency of the core proteins of dermatan sulphate proteoglycans in a variant form of Ehlers-Danlos syndrome. J Intern Med 1989 226 409-416. 

Kawashima H, Hirose M, Hirose J, Nagakubo D, Plaas AH, Miyasaka M. Binding of a large chondroitin sulphate/dermatan sulphate proteoglycan, versican, to L-selectin, P-selectin, and CD44. J Biol Chem 2000 275 35448-35456. 

Schmidtchen A, Frick IM, Bjorck L. Dermatan sulphate is released by protei-nases of common pathogenic bacteria and inactivates antibacterial alpha-defensin. Mol Microbiol 2001 39 708-713. 

B21. Bella, A., Jr., and Danishefsky, I., The dermatan sulphate-protein linkage region. /. Biol. Chem. 243, 2860-2664 (1968). 

F6. Fransson, L.-A., Structure of dermatan sulphate. IV. Glycopeptides from the carbohydrate-protein linkage region of pig skin dermatan sulphate. Biochim. Biophys. Acta 156, 311-316 (1968). 

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