4 ,6 -Diamidino-2-phenylindole, staining

R. E. Hicks, R. I. Amann, and D. A. Stahl, Dual staining of natural bacterioplankton with 4, 6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences, Appl. Environ. Microbiol. 571 2158 (1992). 

Microbiological Studies. Bacterial numbers for the Chesapeake Bay samples and the pure cultures were determined by acridine orange direct counts (AODC) (67). Those for the lakes were determined by epifluorescence (68) using DAPI (4, 6-diamidino-2-phenylindole) with a final stain concentration of 1.0 xg/mL, a process that is described in detail elsewhere (69). 

Cell nuclei are stained with DAPI (4, 6-diamidino-2-phenylindole) (blue, top and bottom), (f) Purmorphamine (2 pM) induces osteogenesis of mouse multipotent... 

Fig. 8.3. Idiogram of chromosomes 1-4 and the sex chromosomes Z and W from the domestic fowl. Chromosomes on the left of each pair are stained with chromomycin, which shows a preference for GC-rich sequences, whereas those on the right are treated with 4,6-diamidino-2-phenylindol-2HCl (Dapi), which preferentially stains AT-rich sequences (Fritschi Stranzinger, 1985).

Immunofluorescence staining combined with CLSM can be applied to visualize cell components (e.g., nucleus-DAPI (6-diamidino-2-phenylindole) or actin filaments-phalloidin) and ECM molecules. 

FIGURE 2.1.8 (a) Design of virus-like polymer particles by self-assembling amphiphilic block copolymer molecules around RNA-based polyelectrolyte complexes, (b) Uptake and intracellular distribution of bilayer siRNA-polyethylenimine (PEI) complexes prepared at different concentrations of copolymer into HeLa cells after 24 h of incubation ([siRNA] = 50 nM). Copolymer and siRNA molecules are labeled with fluorescein and Cy5, respectively. Last column Overlay of DIC (differential interference contrast) and fluorescence images (blue nuclei stained with DAPI [4, 6-diamidino-2-phenylindoleD [108]. 

Figure 15.4 Printed primary human myoblasts and rat tenocytes to generate muscle-tendon tissues for substance testing. The cells were printed and immunostained for myosin heavy chain (green) in (a) and collagen I (green) in (b) for muscle and tendon tissues, respectively. In (c) the close-up of (a) shows the characteristic muscle striation and multinucleated cell bodies of differentiated myoblasts. Nuclei are stained in blue with (4, 6-diamidino-2-phenylindole). In (d) the close-up of (b) shows the characteristic mature tendon collagen I pattern around the cell nuclei, which are stained in red with propidium iodide. Scale for (a) and (b) = 1 mm. Scale for (c) and (d) = 20 pm.

The cells are stained with a fluorochrome. The two most commonly used stains are 4, 6-diamidino-2-phenylindole (DAPl) [113] and acridine orange (AO) [63]. Both of those compounds bind nucleic acid molecules, DNA and RNA. A number of procedures have been developed for each one of dyes in question (e.g., stain, then filter versus filter... 

A similar assay was used to examine the effects of extracts from three soft corals Alcyonium paessleri, Clavularia frankliniana, and Gersemia antarctica, against three species of marine bacteria (Slattery et al. 1995). In this assay, replicate 20-g soft coral samples were extracted in 200 ml of solvent and then diluted to tissue level concentrations. Twenty-five microliters of each soft coral extract or a solvent control were then spotted onto glass microscope slide cover-slips. The coverslips were placed into individual wells of tissue culture plates that contained 2 ml of sterile-filtered seawater seeded with 50 Lil of a bacterial suspension (7.5 x 10 cells/ml). Following a 48-h incubation, the coverslips were washed with sterile-filtered seawater to remove unattached cells and stained with DAPI (4,6-diamidino-2-phenylindole). The number of cells in each of five replicate microscope fields per coverslip were counted using epifluorescence microscopy. Bacterial attachment was found in this study to be inhibited by the chloroform extracts of both A. paessleri and G. antarctica and the methanol extract of G. antarctica. The active chloroform extracts did not display antimicro-... 

Xian, Y Shen, L. Gao, Y Yamauchi, X. Shen, X. M. Ma, N. Comparison of 4, 6 -diamidino-2-phenylindole and Giemsa stainings in preimplantation mouse embryos micronucleus assay including a triple dose study. Irui Health... 

The 16-cell cyst is surrounded by somatically derived epithelial cells (the follicle cells), which eventually divide mitotically four to five times. In a single view through the midsection of an e chamber, the follicle cells stained with DAPl (4, 6-diamidino-2-phenylindole) are seen as brightly staining dots at the perimeter (Figure 4.2). 

Staining with 4,6-diamidino-2-phenylindole (DAPI see Protocol 9.6)... 

DAPI (4, 6-diamidino-2-phenylindole) is a fluorescent dye used to stain DNA. 

Ocarino, N. M. Bozzi, A. Pereira, R. D. O. Breyner, N. M. Silva, V. L. Castanheira, R Goes, A. M. Serakides, R. Behavior of mesenchymal stem cells stained with 4, 6-diamidino-2-phenylindole... 

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