Oligonucleotide probe target

R. E. Hicks, R. I. Amann, and D. A. Stahl, Dual staining of natural bacterioplankton with 4, 6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences, Appl. Environ. Microbiol. 571 2158 (1992). 

Lebaron, P. Catala, P. Fajon, C. Joux, F. Baudart, J. Bernard, L. A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification. Appl. Environ. Microbiol. 1997, 63, 3274-3278. 

Manz, W. In situ analysis of microbial biofilms by rRNA-targeted oligonucleotide probing. Methods Enzymol. 1999,310,79-91. 

Figure 1.57 Base-pairing can occur between complementary bases in opposing oligonucleotide strands. These predictable interactions form the basis for using synthetic oligonucleotide probes to target particular DNA sequences.

Oligonucleotide probes may be labeled with small fluorescent molecules for detection of hybridization by luminescence. Fluorescent probes are widely used in assay systems involving bio-specific interactions (Chapter 9). Receptors for ligands may be localized in tissues or cells by modification of the ligand with the appropriate fluorophore. Targeted molecules may be... 

Fig. 25 AFM images of silicon chip siufaces a cleaned, rehydroxylated silicon surface, b self-assembled MPTS layer on silicon, c immobilised oligonucleotide probe, d surface after hybridisation to a complementary target [119]. Reprinted with permission...

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