Acridine orange direct counting

Microbiological Studies. Bacterial numbers for the Chesapeake Bay samples and the pure cultures were determined by acridine orange direct counts (AODC) (67). Those for the lakes were determined by epifluorescence (68) using DAPI (4, 6-diamidino-2-phenylindole) with a final stain concentration of 1.0 xg/mL, a process that is described in detail elsewhere (69). 

Accelerated pesticide degradation rate, characterization, 273-274 Acridine orange direct counting, 156 Adaptation characterization, 181 definition, 168 mechanisms, 168-174 microbial, See Microbial adaptation occurrence, 168... 

Total Bacteria Count The TBC is determine by directly counting the actual number of microorganisms collected on a filter after it is used to filter a sample of the water in question.5 The sample is stained with acridine orange and viewed with an epi-illuminated fluorescent microscope. This technique is more accurate and quicker than the culture technique, but is not as practical for field work. 

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