D Structural Analysis

Swiderek, K. M. Alpert, A. J. Heckendorf, A. Nugent, K. Patterson, S. D. Structural analysis of proteins and peptides in the presence of detergents Tricks of the trade. ABRF News 1997. 

Earlier work in this field [28] indicated that acetylcholinesterase enzymes would be suitable biomolecules for the purpose of pesticide detection, however, it was found that the sensitivity of the method varied with the type and source of cholinesterase used. Therefore the initial thrust of this work was the development of a range of enzymes via selective mutations of the Drosophila melanogaster acetylcholinesterase Dm. AChE. For example mutations of the (Dm. AChE) were made by site-directed mutagenesis expressed within baculovirus [29]. The acetylcholinesterases were then purified by affinity chromatography [30]. Different strategies were used to obtain these mutants, namely (i) substitution of amino acids at positions found mutated in AChE from insects resistant to insecticide, (ii) mutations of amino acids at positions suggested by 3-D structural analysis of the active site,... 

St. Michael, F., Li, J., Vinogradov, E., Larocque, S., Harper, M., Cox, A.D. Structural analysis of the lipooligosaccharide of Pasteurella multocida strain VP161 identification of both Kdo-P and Kdo-Kdo species in the lipopolysaccharide. Carbohydr Res 340 (2005b) 59-68. 

Parthiban V, Gromiha MM, Hoppe C, Schomburg D. Structural analysis and prediction of protein mutant stabihty using distance and torsion potentials role of secondary structure and solvent accessibility. FYoteins. 2007 66 41-52. 

In the state of G-actin, the bound nucleotide molecule, ATP or ADP, is quickly exchangeable with the free molecule in solution. An equilibrium is established between bound and free molecules. On the other hand, in F-actin the bound nucleotide molecule is not easily exchangeable. According to the 3-D structural analysis, G-actin appears to be composed of four domains. ATP or ADP is found in a deep cleft in the middle of the molecule. Probably, in G-actin the cleft has some flexibility to expose bound ATP or ADP to the solvent, whereas in F-actin such flexibility is limited. Therefore, whether each actin monomer in F-actin has ATP or ADP depends on the history of the monomer. 

Quin L D and Verkade J G (eds) 1994 Phosphorus-31 NMR Spectral Properties in Compound Characterization and Structural Analysis (New York VCH)... 

Di stefano D L and Wand A J 1987 Two-dimensional NMR study of human ubiquitin a main chain directed assignment and structure analysis Biochemistry 26 7272-81... 

A. W. Norman, R. Bouillon, and M. Thomasset, eds.. Vitamin D, Structure, Function Analysis and Clinical Application, Proceedings of the Eighth Workshop on Vitamin D, Paris, July 5, 1991, Walter de Gmyter, Berlin, 1991. 

Thieno[3,2-d]thiazolin-4-one, 2-acetamido-synthesis, 5, 138 Thieno[3,4-d]thiepins X-ray structure analysis, 7, 558 Thieno[2,3-h]thiinium perchlorate, 2,4-dimethyl-synthesis, 4, 1028... 

Wang, D., Bode, W., Huber, R. Bovine chymotrypsinogen A. X-ray crystal structure analysis and refinement of a new crystal form at 1.8 A resolution. /. Mol. Biol. 

Field ion microscopy, scanning tunnelling microscopy (morphology analysis, etc.) L.E.E.D. (structure)... 

Saito et al. achieved the first direct confirmation of double alkylation of purine bases by azinomycin B [140]. They incubated azinomycin B with the self-comple-mentary DNA duplex d(TAGCTA)2 and monitored the reaction by HPLC and ion spray MS. They observed initial formation of a monoadduct that was then converted into a crosslinked bisadduct. The crosslink position was identified as between the guanine of one strand and the 5 -adenine on the other strand by thermo-lytic depurination. Further decomposition prevented structural analysis of the azi-... 

The ground states of P/ [26, 27] and As " [28] have D structures. Molecular orbital analysis revealed that the square planar dianion exliibits the characteristic... 

The absolute configuration of (—)-29 was established by X-ray crystal structure analysis of the bromolactone (89), which was prepared from (—)-29 by bromolactonization with hypobromous acid. It was found that ( )-29 belongs to the d series of carba-sugars, and hence, (+)-29 corresponds to the L series. - ... 

Shea, E.M., Gibeaut, D.M., and Carpita, N.C. (1989) Structural analysis of the cell walls regenerated by carrot protoplasts. Planta, 179 293-308. 

One of the best-studied carrier molecules is produced as a primary excretory constituent of the adult male mouse, known from its consistent high concentration as the major urinary protein (MUP). The basic 3-D structure of the protein was initially obtained from a monoclinic crystal of recombinant protein (MUP-I), constructed by induction in a bacterial expression system and purified to homogeneity (Kuser, 1990). A wild type version of MUP finally yielded to NMR analysis a clone of the r-isoform (162 residues) was labelled and compared with the crystal-structure (Lucke et al., 1990). Two views of the molecule... 

Both positional linkages (uronic acid to hexosamine and hexosamine to uronic acid) were established as being (1 — 4) by structural analysis of the previously mentioned (see Section IV), crystalline disaccharides containing D-glucuronic acid, isolated from an acid hydrolyzate of carboxyl-reduced heparin.128-129 Further evidence was obtained from the structure of the D-glucuronic acid-containing counterpart of disaccharides 6 and 8, obtained as minor products from pig-mucosal heparin following nitrous acid deamination,1110 136-138 and acid hydrolysis followed by N-acetylation,130 respectively. 

P212121 Z = 4 D = 1.38 R = 0.04 for 1,707 intensities. The eno-pyranosyl group has the °HS conformation, with Q = 49 pm, 0=52°, (p = 320°. This conformation is very similar to that of Ci2H1806 (EACXHP10)67 and CuH1606 (THHXPY).60 The twist about the ring C=Cbond is 3°. The C-P bond distance is 181.4 pm. The results of the crystal-structure analysis were correlated with a detailed analysis of the -n.m.r. spectrum. 

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