Cuvettes disposable

Roche COBAS MIRA variable 125 30 2-95 37 AMW,P disposable cuvettes... 

TEMPERATURE CONTROL, WB WATER, OB OIL BATH, HAB>HOT AIR BATH, E ELECTRIC CUVETTE, P-PLASTIC, D DISPOSABLE, FT FLOW THROUGH DISPLAY, D DIGITAL, A ANALOG COMPUTER AND DATA REDUCTION, +=YES... 

The use of disposable plastic cuvettes is recommended. If glass cuvettes are used, remove adhered protein-dye complex on the walls with 96% ethanol or methanol. 

Materials commonly use as solid supports include polystyrene, polyvinyl, nylon, glass, nitrocellulose, silica, polyacrylamide, or polystyrene beads. Separation of the bound from the free reagents can be achieved through either filtration for particulate solid supports such as agarose, polyacrylamide, and polystyrene beads, or centrifugation. For disposable forms of solid supports such as multiwell plates, plastic tubes, cuvettes, balls, and dipsticks, separation can be performed through simple rinsing steps. 

Disposable cuvette cartridges for optical measurements have been developed (49). The cartridge is formed from three flexible thin layers. The middle layer is an adhesive sheet with cut out regions, typically between 30 and 50, serving as optical chambers bounded... 

L.J. Dietz and C.E. Todd, Disposable optical cuvette cartridge with low fluorescence material, US Patent 7 259 845, assigned to PPD Biomarker Discovery Sciences LLC (Wilmington, NC), August 21, 2007. 

Glass cuvettes are the least expensive but, because they absorb UV light, they can be used only above 340 nm. Quartz or fused silica cuvettes may be used throughout the UV and visible regions ( 200-800 nm). Disposable cuvettes are now commercially available in polymethacrylate (280-... 

Using glass cuvettes, measure the absorption spectrum of each fraction from 400 to 700 nm. A cuvette containing acetone should be used as reference. A Pasteur pipet may be used to transfer solutions to and from the cuvette. After all spectra have been obtained, dispose of the acetone solutions in the waste organic container in the laboratory. 

Disposable polystyrene cuvettes eliminate thejob of cleaning dye-stained quartz or glass cuvettes. 

Disposable plastic cuvettes are more convenient to use in spectrophotometric detection however, plastic melts after extended exposure to strong acids. In addition, for more linearity and accuracy in the measurements, many important parameters must be considered. It is advantageous to use cuvettes with path lengths of (0.01 to 0.5 cm), because they use a smaller volume of sample and often give more reproducible results. 

Transfer 1 ml from each tube into a disposable cuvette. 

Transfer 1 ml supernatant from each tube into a disposable cuvette and measure the absorbance at 510 nm. 

Disposable plastic cuvettes should not be used, as the acetone will solubilize the cuvette and cause turbidity in the reference cell. 

Pipet 1.0 ml of 40 mM sodium phosphate buffer, pH 6.8, into a disposable cuvette and place it in the sample port of the spectrophotometer. 

Cold room (4°C) or cold box Computer (PC or Macintosh) and printer Conical centrifuge tubes, 15- and 25-ml plastic Cuvettes, plastic disposable, glass, and quartz Darkroom and developing tank, or X-Omat automatic X-ray film developer (Kodak) Desiccators (including vacuum desiccators) and desiccant Dry ice... 

In a few cases, analyses may be done on the process floor using a conventional scanning spectrophotometer and the usual laboratory cuvette or a specially made vial that fits into the sample cavity of the instrument. Cuvettes may be made from quartz (various grades depending upon UV transmission requirements), or from plastic (disposable) when UV wavelengths are not required for the measurements. In most cases these at-line analyzers have lower performance characteristics than dedicated laboratory instruments, but may be quite adequate for the task at hand. However, their use still requires manual sampling... 

A number of commercial machines are available and cuvettes may be bought or constructed from disposable spectrophotometer cuvettes into which aluminium electrodes are glued. They can be sterilised with 70% ethanol and then washed with sterile PBS. (This is also true for the disposable commercial cuvettes.) The same apparatus can also be used for cell fusion (electrofusion) (see 13.7.3 and Glassy, 1988). 

In other disposable cuvettes, prepare different solutions of -galactosidase from the stock solution. The following enzyme dilutions should be carried out for a final volume of 2 ml 1/10,1/15, 1/20,1/25,1/30, 1/40, and 1/50. ... 

Disposable cuvettes are used only once to eliminate the possibility of lysozyme carryover. All microcentrifuge tubes and pipette tips are autoclaved, and buffers and BSA solutions are not used beyond 7 days. The M. lysodeikticus suspension is prepared 18-24 hr before use and set at 37° with shaking, to hydrate fully. It was found that substrate prepared just before use settled much faster and interfered with the signal at the low concentrations of enzyme used. 

PopeBca BR, Mfller DM, Holen JT, et al. Fluorescence polarization immunoassay II. Analyzer for rapid, precise measurement of fluorescence polarization with use of disposable cuvettes. Clin Chem 1981 27 1198-201. 

An HTS assay typically requires the detection of minute amounts of a probe at nanomolar or lower concentrations in the presence of a 10,000-fold or higher excess of other reagents chemical test compounds from the screening Hbrary at a level of 10-100 pm large amounts of proteins and lipids from cells or crude membranes containing only fractions of the target and a sample compartment (micro-titer plate), which is a disposable made of plastic and generates a totally different situation to a quartz cuvette in a spectroscopic set-up. 

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