Microcentrifuge tube

Incubate specific mouse monoclonal primary antibody with FITC -conjugated mouse IgG specific Fab fragments at a ratio of 1 2 (weight for weight, based on concentration data supplied by manufacturers) in a small volume (e.g., in 10 pi or more, typically 1 pg of primary antibody in 10 pi) of staining buffer in a microcentrifuge tube for 20 30 min at room temperature. 

Using a micropipettor, add 10 pL of the reaction buffer to a small reaction vial or microcentrifuge tube. Add 15 pL of DNA. Then, with a fresh micropipet tip, add 15 pL of one enzyme. 

A series of microcentrifuge tubes is set up, containing the membranes (usually 0.1-0.5 pM in receptor binding sites approximately 0.05-0.25 mg protein/ml) in Torpedo membrane protein and [ H]acetyl-choline concentrations ranging from 0 to 2.0 pM. Nonspecific binding is determined in the presence of excess unlabelled acetylcholine (100 pM-1 mM). 

Mix and transfer the contents of each microwell to a 1.5-ml microcentrifuge tube. 

Brumback [28] has described a custom robotic system designed to automate the extraction of proteins from plant samples. Leaf or callus material (5—25 mg) is presented to the robot in microcentrifuge tubes, the system performs buffer dispensing, grinding, centrifugation, and pipetting unit operations, and a cleared supernatant is delivered in a 96-well microassay plate format for subsequent analysis. 

RIA Procedure. The RIA was developed using an antiserum pool which contained 2.0 mg/ml of anti-STXOL antibody. The protocol was designed for use in a 1.5 ml microcentrifuge tube. 

This assay was developed to be completely performed in a microcentrifuge tube. The basic procedure involves addition of a... 

Ligate vector and gene product To a microcentrifuge tube add ... 

Sterile microcentrifuge tubes Sterile pipettes and pipette tips SDS-polyacrylamIde gel electrophoresis system... 

A sterile plastic box that can accommodate six 100 ml tissue culture plates Microcentrifuge tubes Sterile pipette tips Microscope... 

Remove an agarose plug over the plaque using a sterile pipette tip and place in a microcentrifuge tube containing... 

Transfer 2 x 1.5 ml of the culture to microcentrifuge tubes and separate cells from the medium by centrifugation at 2500 g for 5 min for analysis by SDS-PAGE. 

Dilute the stock solution of Silencer Select GAPDH siRNA or Silencer Select negative control siRNA to a concentration of 100 pmol/500 pi in Opti-MEM I in a microcentrifuge tube. 

Dilute the peptide stock solution in Opti-MEM I Reduced Serum Medium to 500 pi in a microcentrifuge tube so that the weight ratio of peptide to siRNA (prepared in step 1) is 10 to 1 (see Notes 3 and 4). 

Mix the suspension by gently inversing the microcentrifuge tube several times. The mixture is incubated at room temperature for 30 min to allow the formation of stable peptide/ siRNA complexes. 

An aliquot of Ac-AKEAAHAEAAEAA-NH, in H,0 was placed in a preweighed 1.5-mL microcentrifuge tube and lyophilized to dryness. The dried powder (15 mg, 11.7 pmol) was dissolved in dry DMSO (100 pL) and 29 pM bis(p-nitrophenyl) ester of iron(III) mesoporphyrin IX in DMSO (100 pL) was added, followed by DIPEA (45 pL, 22 equiv). The soln was warmed to 40 °C until HPLC indicated no further reaction (ca. 3h). It was then diluted to 1.0 mL with 50 mM NaOAc, pH 6, and purified by sequential ion-exchange chromatography (Sephadex CM50 50 mM NaOAc, pH 6), RP-HPLC (Vydac... 

Obtain a precast SDS-polyacrylamide slab gel or prepare one according to instructions in Experiment 4. The recommended gel is 12°/o acrylamide with a thickness of 0.75 mm. Protein samples are prepared as follows Purified proteins (transferrin, bovine serum albumin, a, -antitrypsin, a-lactalbumin from Experiment 4, and molecular weight standards) are supplied in Tris buffer, pH 6.8 solutions at a concentration of 1 mg/mL. Sera samples have been diluted and are ready for use. Prepare protein samples for electrophoresis in 0.5-mL microcentrifuge tubes with attached caps. Label the tubes from 1 to 5 as below or per your Instructor s directions. 

Obtain a small microcentrifuge tube for each reaction mixture and add the following components ... 

Anesthetize animals (see Note 1), and take a blood sample from the jugular or tail vein into a capped 0.5- or 1.5-mL microcentrifuge tube to act as a preimmune sample. Allow it to clot, centrifuge at 1500g, remove the serum, and store at -20°C... 

In six separate microcentrifuge tubes, mix 7 il four-enzyme cocktail with 0.5 ml of 50 mM Tris-Cl, pH 8.0. 

Place the excised membrane band in a 1,5-ml microcentrifuge tube containing 0.2 to 0.5 ml of Triton/SDS elution buffer for every square centimeter of membrane. 

Remove supernatant, place it in a new microcentrifuge tube, and microcentrifuge again to remove any particulate material. 

Rinse the desired number of 1,5-ml microcentrifuge tubes (two tubes per sample) three times with extraction solution, and air dry under an aluminum foil dust cover. 

Place pieces of membrane containing the protein of interest in a clean microcentrifuge tube. 

Combining several bands from multiple gels is usually necessary. Use a pair of tweezers cleaned with 100% methanol to transfer the membrane pieces to the microcentrifuge tube. Avoid contaminating the membrane pieces and microcentrifuge tube with airborne dust or residues from fingers. 

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