Cultured hippocampal neurons

Saez ET, Pehar M, Vargas MR, Barbeito L, Maccioni RB (2006) Production of nerve growth factor by beta-amyloid-stimulated astrocytes induces p75NTR-dependent tau hyperphosphorylation in cultured hippocampal neurons. J Neurosci Res 84 1098-1106 Sala C, Roussignol G, Meldolesi J, Fagni L (2005) Key role of the postsynaptic density scaffold proteins Shank and Homer in the functional architecture of Ca homeostasis at dendritic spines in hippocampal neurons. J Neurosci 25 4587 592 Santello M, Volterra A (2008) Synaptic modulation by astrocytes via Ca(2-l-)-dependent glutamate release. Neuroscience 158 253-9... 

Piller SC, Jans P, Gage PW, Jans DA (1998) Extracellular HIV-1 virus protein R causes a large inward current and cell death in cultured hippocampal neurons implications for AIDS pathology. Proc Natl Acad Sci USA 95 4595-4600... 

Analysis of mRNA Translation in Cultured Hippocampal Neurons 143... 

The cultured hippocampal neurons infected with a control lentivirus or one expressing a short hairpin RNA against CPEB3 (siCPEB3- 4) for... 

Kristiansen, U., Lambert, J. D., Falch, E and Krogsgaard-Larsen, P. (1991) Electrophysio-logical studies of the GABAA receptor ligand, 4-PIOL, on cultured hippocampal neurones. Br. J. Pharmacol. 104, 85-90. 

PIOL has previously been characterized as a partial agonist in whole-cell patch-clamp studies on cerebral cortical neurones and cultured hippocampal neurones (15,25). Only the methyl and ethyl analogs, 1 and 2, retained detectable ability to induce an agonist effect as shown on cultured cerebral cortical neurones and recombinant receptors (24). In the study of recombinant GABAa receptors, the weak responses of 1 and 2 were potentiated by simultaneously administered lorazepam and inhibited by the competitive antagonist SR 95531. 

Gibbs, J.W., Sombati, S., DeLorenzo, R.J., and Coulter, D.A. (2000) Cellular actions of topiramate blockade of kainate-evoked inward currents in cultured hippocampal neurons. Epilepsia 41 S10-S16. 

Pereira EFR, Reinhardt-Maehcke S, Schrattenholz A, et al Identification and functional characterization of a new agonist site on nicotinic acetylchohne receptors of cultured hippocampal neurons. J Pharmacol Exp Ther 265 1474-1491, 1993 Perez de la Mora N, Hemandez-Gomez AM, et al Cholecystokinin-8 increases K -evoked [ H] gamma-aminobutyric acid release in shces from various brain areas. Eur J Pharmacol 250 423-430, 1993... 

In order to confirm the possible interaction of ethanol and crocin on NMDA receptors, we also performed whole-cell patch recording with primary cultured hippocampal neurons and measured membrane currents induced by the application of NMDA in a voltage-clamped condition. Application of 100 pM NMDA induced an inward current of 100.2 9.8 pA (n=10) at a holding potential of -60 mV. The NMDA-induced inward current was not affected by 10 pM CNQX (data not shown), but was completely abolished by 30 pM APV, supporting the fact that the response was mediated by NMDA receptors. Ethanol inhibited NMDA-induced currents in a concentration-dependent manner. Crocin (10 pM) had no effect on NMDA-induced currents by itself (data not shown), but attenuated the inhibitory effect of ethanol on NMDA-induced currents. The concentration-effect curve for ethanol was shifted to the right by the presence of crocin [22]. 

Kahlert S., Zundorf G., and Reiser G. (2005). Glutamate-mediated influx of extracellular Ca2+ is coupled with reactive oxygen species generation in cultured hippocampal neurons but not in astrocytes. J. Neurosci. Res. 79 262-271. 

Mattson M. P. (1990). Antigenic changes similar to those seen in neurofibrillary tangles are elicited by glutamate and Ca2+ influx in cultured hippocampal neurons. Neuron 2 105-117. 

Resveratrol, an active silbene from grapes, was shown to concentration-depend-ently protect against AP-induced toxicity in cultured hippocampal neurons. The mechanisms involved in the neuroprotective effects of resveratrol likely include PKC as revealed by the inhibitory action of the potent PKC antagonist GF 109203X and... 

Bastianetto, S., Zheng, W.H., and Quirion, R. Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide-related toxicity in cultured hippocampal neurons. Br J Pharmacol, Oct, 131(4), 711-720, 2000. 

Crump FT, Fremeau RT, Craig AM (1999) Localization of the brain-specific high-affinity 1-proline transporter in cultured hippocampal neurons molecular heterogeneity of synaptic terminals. Mol Cell Neurosci 13 25-39. 

Terbush DR, Holz RW (1990) Activation of protein kinase C is not required for exocytosis from bovine adrenal chromaffin cells. The effects of protein kinase C(19-31), Ca/CaM kinase 11(291— 317), and staurosporine. J Biol Chem 265 21179-84 Tesmer VM, Kawano T, Shankaranarayanan A et al (2005) Snapshot of activated G proteins at the membrane the Galphaq-GRK2-Gbetagamma complex. Science 310 1686-90 Thakur P, Stevens DR, Sheng ZH et al (2004) Effects of PKA-mediated phosphorylation of Snapin on synaptic transmission in cultured hippocampal neurons. J Neurosci 24 6476-81 Thompson SM, Capogna M, Scanziani M (1993) Presynaptic inhibition in the hippocampus. Trends Neurosci 16 222-7... 

Kirk IP, Richardson PJ (1994) Adenosine A2a receptor-mediated modulation of striatal [3H]GABA and [3H]acetylcholine release. J Neurochem 62 960-6 Koch H, von Ktigelgen I, Starke K (1997) P2-receptor-mediated inhibition of noradrenaline release in the rat hippocampus. Naunyn-Schmiedeberg s Arch Pharmacol 355 707-15 Koch H, von Ktigelgen I, Starke K (1998) P2-receptor-mediated inhibition of noradrenaline release in the rat pancreas. Naunyn-Schmiedeberg s Arch Pharmacol 357 431 10 Koizumi S, Inoue K (1997) Inhibition by ATP of calcium oscillations in rat cultured hippocampal neurones. Br J Pharmacol 122 51-8... 

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