Sertoli cells culture

Clearly, major differences seem suggested in the mechanisms of hormone-membrane receptor interactions leading to various post-binding events [11], For FSH, as an example, there have been reports of hormone effects on membrane potentials of cultured Sertoli cells [12] and on conductance of artificial lipid membranes [13], as well as on amino acid transport systems [14]. Indeed, there has been one report of receptor-mediated gonadotropin action without receptor occupancy... 

Kato, R., Maeda, T., Akaike, T, and Tamai, I. (2005) Nucleoside transport at the blood-testis barrier studied with primary-cultured sertoli cells. The Journal of Pharma cology and Experimental Therapeutics, 312 (2), 601-608. 

F., and Czech, M. P., the actions of insulin-like growth factors I and II on cultured Sertoli cells. Endocrinology (Baltimore) 114, 240-246 (1984). 

Sievertsson, H., Fryklund, L., Uthne, K., Hall, K., and Westermark, B., Isolation and chemistry of human somatomedins A and B. Ado. Metah. Disord. 8, 47-60 (1975). Skinner, M. K., and Griswold, M. D., Multiplication stimulating activity (MSA) can substitute for insulin to stimulate the secretion of testicular transferrin by cultured Sertoli cells. Cell Biol. Int. Bep. 7, 441-446 (1983). 

Shingleton JL, Skinner MK, Ong DE (1989) Characteristics of retinol accumulated from serum retinol-binding protein by cultured Sertoli cells. Biochemistry 28 9641-9647... 

Bishop PD, Griswald MD (1987) Uptake and metabolism of retinol in cultured Sertoli cells evidence for a kinetic model. Biochemistry 26 7511-7518... 

Chapin RE, Phelps JL, Burka LT, et al. 1991. The effects of tri-ozt/zo-cresyl phosphate and metabolites on rat Sertoli cell function in primary culture. Toxicol Appl Pharmacol 108 194-208. 

Williams J, Foster PM. 1989. The effects of 1,3-dinitrobenzene and mono-(2-ethylhexyl) phthalate on hormonally stimulated lactate and pyruvate production by rat Sertoli cell cultures. Toxicol Lett 47 249-257. 

Heindel, J.J. Powell, C.J. (1992) Phthalate ester effects on rat Sertoli cell function in vitro effects of phthalate side chain and age of animal. Toxicol appl. Pharmacol, 115, 116-123 Hellwig, J., Freudenberger, H. Jackh, R. (1997) Differential prenatal toxicity of branched phthalate esters in rats. Food Cosmel Toxicol, 35, 501-512 Hildebrand, H., Schmidt, U., Kempka, G, Jacob, R., Ahr, H.J., Ebener, C., Goretzki, RE. Bader, A. (1999) An in-vitro model for peroxisome proliferation utilizing primary hepatocytes in sandwich culture. Toxicol. In Vitro, 13, 265-273... 

Janecki A Steinberger A (1986) Polarized Sertoli cell functions in a new two-compartment culture system. J Androl, 7(1) 69-71. 

Tres LL, Smith EP. Van Wyk KK, Kierszenbaum AL (1986) Immunoreactive sites and accumulation of somatomedin-C in rat Sertoli-spermatogenic cell co-cultures. Exp Cell Res, 162(1) 33-50. 

Chapin RE, Gray TJ, Phelps JL, et al. 1988. The effects of mono-(2-ethylhexyl)-phthalate on rat Sertoli cell-enriched primary cultures. Toxicol Appl Pharmacol 92 467-479. 

Grasso P, Heindel JJ, Powell CJ, et al 1993. Effects of mono(2-ethylhexyl) phthalate, a testicular toxicant, on follicle-stimulating hormone binding to membranes from cultured rat Sertoli cells. Biol Reprod 48 454-459. 

Heindel JJ, Chapin RE. 1989. Inhibition of FSH-stimulated cAMP accumulation by mono(2-ethylhexyl) phthalate in primary rat Sertoli cell cultures. Toxicol Appl Pharmacol 97 377-385. 

Lloyd SC, Foster PMD. 1988. Effect of mono-(2-ethylhexyl)phthalate on follicle-stimulating hormone responsiveness of cultured rat Sertoli cells. Toxicol Appl Pharmacol 95 484489. 

S.C.J. Reader, and R.M.D. Foster, The in vitro effects of four isomers of dinitro-toluene on rat sertoli and sertoli-germ cell co-cultures Germ cell detachment and lactate and pyruvate production. Toxicol. Appl. Pharmacol. 106 287-294, 1990. 

Inhibin, an ovarian protein hormone, has been shown to selectively decrease FSH but not LH secretion by the anterior pituitary cell. The gonadal source of inhibin has been localized to the testis Sertoli cells [76] and ovarian granulosa [77]. In cultured granulosa cells, FSH increases inhibin production via stimulation of the protein kinase A pathway. In vivo treatment with injections of gonadotropin also increases the circulating levels of inhibin [78], whereas treatment with inhibin... 

There are methods for culturing various cells from the male reproductive system, such as pituitary cells, Sertoli cells, and germ cell-Sertoli cell cocultures. Although these investigations help elucidate mechanisms of action, they cannot by themselves generate sufficient evidence of reproductive toxicity. 

Perez-Infante V, Bardin CW, Gunsalus GL, Musto NA, Rich KA Mather JP (1986) Differential regulation of testicular transferrin and androgen binding protein secretion in primary cultures of rat Sertoli cells. Endocrinology 118 383-392. 

Steinberger, A., and Clinton, J. P. (1993). Two-compaitment cultures of Sertoli cells — Applications in testicular toxicology. In Methods in To.xicology (part A). Male Reproductive Toxicology (R. E. Chapin and J. J. Heindcl, Eds.), pp. 230-245. Academic Press, New York. 

Since neoplastic transformation is closely related to cytoskeletal changes, Panagopoulou et al. (2002) developed a culture system of purified populations of Sertoli cells from 20-d-old rats. After the addition of 10" M 12-0-tetradecanoylphorbol-13-acetate Sertoli cells began to round up and their cytoplasm retracted towards a central region. Actin bundle organisation was disrupted and vinculin assumed a punctuate distribution throughout the cell. 

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