Spermatogenic cells

Xiao H, Zhang XC, Zhang L, Dai XQ, Gong W, Cheng J, Gao R, Wang X (2006) Fenvalerate modifies T-type Ca2+ channels in mouse spermatogenic cells. Reprod Toxicol 21 48-53... 

Binding of Hg2 + to DNA may cause degeneration and chromosomal breakage of spermatogenic cells [235]. Moreover, mercuric chloride gives a direct inhibition of sequence synthesis in murine spermatogonia cells and possibly an inhibition of essential spermatogenic enzymes [236]. 

Shiratsuchi, A., Umeda, M., Ohba, Y., and Nakanishi, Y., 1997, Recognition of phosphatidylserine on the surface of apoptotic spermatogenic cells and subsequent phagocytosis by Sertoh cells of the rat. J. Biol. Chem. 272 2354-2358. 

What is special about the testis that requires the absence of the circadian clock so pervasive in other tissues The testis has a number of characteristics, which make it quite different from other tissues. The testis contains spermatogenic cells that perform a constant and complex cell differentiation program where reductive cell divisions occur. It may be that the complex pattern of gene expression engendered by the circadian clock leads to unfavourable interactions with the developmental process of paramount importance to the testis. Alternatively, the normal oscillation of clock gene expression may be distorted by other transcriptional regulators or co-activators which are only present in seminiferous tubules (Sassone-Corsi 2002). 

Tres LL, Smith EP. Van Wyk KK, Kierszenbaum AL (1986) Immunoreactive sites and accumulation of somatomedin-C in rat Sertoli-spermatogenic cell co-cultures. Exp Cell Res, 162(1) 33-50. 

Yamazaki, Y., Fujimoto, H., Ando, H., Ohyama, T., Hirota, Y. and Noce, T. (1998) In vivo gene transfer to mouse spermatogenic cells by deoxyribonucleic acid injection into seminiferous tubules and subsequent electroporation. Biol. Reprod., 59, 1439-1444. 

Beebe LE. 1990. Adenylate cyclase regulation in the spermatogenic cell plasma membrane Modulating effects of TP A and TCDD [Abstract], Diss Abstr Int [B] 50 2860... 

Rat (Wistar) 15 d (GO) 2000 (decreased testicular weight, changes in tubules, damaged spermatogenic cells reduced sperm count) Parmaretal. 1987... 

Sotomayor, R.E., G.A. Sega, and R.B. Cumming. Unscheduled DNA synthesis in spermatogenic cells of mice treated in vivo with the indirect alkylating... 

A micronucleus assay can be conducted with bone marrow cells, peripheral lymphocytes or germ cells (spermatids) of mice. Rats can be used for micronucleus assays with bone marrow or spermatogenic cells but the use of peripheral lymphocytes requires surgical removal of the spleen, since this is the predominant site of removal of micronucleated erythrocytes in rats. 

Trevino, C.L., De la Vega-Beltran, J.L., Nishigaki, T, Eelix, R., and Darszon, A. 2005. Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction. J Cell Physiol, Epub. 

Oral administration of hexachlorophene to rats causes degeneration of spermatogenic cells. In sheep, 2500 mg kg followed 2 days later by a dose of 50 mg kg also caused extensive damage to spermatogonia after 21 days there was neither sperm in epididymis nor spermatogenesis. 

Georgellis A, Parvinen M, Rydstrom J. 1989. Inhibition of stage-specific DNA synthesis in rat spermatogenic cells by polycyclic aromatic hydrocarbons. Chem Biol Interact 72(1-2) 79-92. 

T channel antagonists inhibit the sustained ZP3-dependent elevation of Ca i in a concentration-dependent fashion that is similar to their potency in inhibiting the T current of spermatogenic cells (Arnoult et al., 1996b). 

Amoult, C., Villaz, and Florman, H.M. (1998). Pharmacological properties of the T-type calcium current of mouse spermatogenic cells. Mol. Pharmacol. 55 1104-1111. 

Hagiwara, S. and Kawa, K. (1984). Calcium and potassium currents in spermatogenic cells dissociated from rat seminiferous tubules. J. Physiol. 556 135-149. 

Karaik, N.S., Newman, S., Kopf, G.S., and CJerton, G.L. (1992). Developmental expression of G protein a subunits in mouse spermatogenic cells Evidence that G , is associated with the developing aerosome. Dev. Biol. 752 393-402. 

Lievano, A., Santi, C.M., Serrano, J., Trevino, C.L., Bellve, A.R., Hemandez-Cruz, A., and Darszon, A. (1996). T-type Ca channels and expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction, FEES Lett. iSS 150-154. 

Schalling, M., Persson, H., Pelto-Huikko, M., Odum, L., Ekman, P., Gottlieb, C., Hokfelt, T., and Rehfeld, J.F. (1990). Expression and localization of gastrin messenger RNA and peptide in spermatogenic cells. J. Clin, Invest. 86 660-669. 

Trevino, C.E., Serrano, C.J., Beltran, C., Felix, R., Darszon, A. (2001) Identification of mouse trp homologs and lipid rafts from spermatogenic cells and sperm. FEBS Lett. 509, 119-125. 

Cytoplasmic Poly(ADP-Ribose) Synthetase in Rat Spermatogenic Cells... 

The enzyme has been found ubiquitously in the nuclei of a great variety of eukaryotic cells, including human [11] and plant [12]. However, three previous studies have presented evidence that the enzyme is also localized in the cytoplasm. Roberts et al. [13] have found the enzyme associated with polysomes and ribosomes of HeLa cells. A poly(ADP-ribose) synthetase was also reported to occur in rat liver mitochondria [14]. More recently we found a significant level of enzymatic activity associated with testis mitochondria [15, 16]. The present paper is a follow-up of this study and it presents evidence that after the nuclear fraction, the most important level of poly(ADP-ribose) synthetase in spermatogenic cells is associated with the micro-somal-ribosomal fraction. 

---