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Culture medium, roots

Addition of other fractions IPN4-IPN14 to the culture medium resulted in activation of root development in BTCL explants to different extent (Table 1). [Pg.697]

A. D. Boulter, J. J. Jeremy, and M. Wilding, Amino acids liberated into the culture medium by pea seedling roots. Plant and Soil 24 121 (1966). [Pg.127]

Increasing concentrations of PVP 360,000 up to 1.0 g L 1 improved antibody accumulation in hairy root culture medium however, above this concentration there was no further increase in antibody levels [19]. Addition of PVP after extracellular foreign protein levels had decreased during plant suspension culture did not result in a recovery of the protein [66]. [Pg.32]

Fig. 2.3 HPLC analysis of ginsenosides recovered from the spent medium of Trichoderma hamatum and Pythium irregulare isolates. A ginsenoside mixture extracted from 3-year-old ginseng roots was added to the culture medium of both T. hamatum and Pythium irregulare, recovered after several days of incubation and analyzed by HPLC. The profile of ginsenosides added to the culture medium (a) included ginsenosides Rgi, Re, Rbi, Rba, Rc, Rd, and F2, as well as gypenoside XVII (G-XVII). (b-e) Profiles of ginsenosides recovered from T. hamatum isolate 3-323 (b), and Pythium irregulare isolates BR 486 (c), BR 598 (d), and BR 1068 (e). Fig. 2.3 HPLC analysis of ginsenosides recovered from the spent medium of Trichoderma hamatum and Pythium irregulare isolates. A ginsenoside mixture extracted from 3-year-old ginseng roots was added to the culture medium of both T. hamatum and Pythium irregulare, recovered after several days of incubation and analyzed by HPLC. The profile of ginsenosides added to the culture medium (a) included ginsenosides Rgi, Re, Rbi, Rba, Rc, Rd, and F2, as well as gypenoside XVII (G-XVII). (b-e) Profiles of ginsenosides recovered from T. hamatum isolate 3-323 (b), and Pythium irregulare isolates BR 486 (c), BR 598 (d), and BR 1068 (e).
Rhizosecretion is easy to scale up and very cost effective with respect to isolation and purification. However, the bioreactor systems used for hairy root cultures differ from those used for plant cell suspensions. Traditional bioreactor systems have recently been adapted for root culture, and this technology is now being taken to commercial scales. The most traditional system is the airlift bioreactor used for microorganisms or plant cells. This system is adapted for the culturing of roots in liquid medium. Mist culture systems have also been developed. For this technology, the volume of the culture medium is reduced and the concentration of the secreted therapeutic protein is increased. If the protein to be produced is known to be quite stable, then a less expensive hydroponic culture can be designed in a manner suitable for scale-up. [Pg.132]

Swainsonine can be produced from root cultures of Swainsona galegifolia (with a maximum concentration of 122,9 pg of swainsonine per gram of dry weight roots) [109,110] or by fermentation of the fungus Metarhizium anisoplilae, which can lead to as much as 500 mg per litter of culture medium [111,112]. [Pg.255]

The adventitious root cultures of Datura innoxia, Duboisia hybrid M-II-8-14 (a cross-bred between D. myoporoides and D. leichhardtii), and Scopolia tangutica were established from the axenic shoot cultures or intact plants (in the case of Duboisia) on MS solid medium containing 0.1 mg/1 NAA or 1.0 mg/1 lAA. The adventitious roots were maintained in MS liquid medium containing the same phytohormones (0.1 mg/1 NAA or 0.5 mg/1 lAA) in the dark. Addition of auxin in the culture medium has been employed for the maintenance of adventitious root cultures [14], however, the adventitious roots of H. albus and H. niger were induced and maintained in hormone-free 1/2 MS medium [15]. [Pg.401]

The time course of alkaloid production were also examined with different concentrations of lAA (0.5 mg/1), 4-Cl-IAA (0.1 mg/1) and 5,6-Cl2-IAA (0.01 mg/1) (Fig. 4). The alkaloid yield in roots cultured with 4-Cl-IAA and 5,6-Cl2-IAA increased more rapidly than those with lAA after two weeks of culture. Scopolamine was also detected in the culture medium, especially after four to five weeks, and its yield increased significantly in the presence of 4-Cl-IAA and 5,6-Cl2-IAA as compared to lAA. A continual recovery of useful secondary metabolites from a culture medium might be important for the industrial production. These results indicate that lAA derivatives (4-Cl-lAA and 5,6-Cl2-IAA) may be applied to the industrial production of scopolamine in cultures. [Pg.404]

The influence of phytohormones and light on the production of tropane alkaloids in transformed roots of Hyoscyamus albus was investigated [25]. In adventitious roots cultured in the dark, the addition of lAA (up to 4 mg/1) to the culture medium only slightly affected alkaloid production (Fig. 8a). On the other hand, when the adventitious roots were cultured under light, the alkaloid content decreased with the addition of lAA. Any combination of lAA with kinetin rapidly... [Pg.408]

Since Kamada etal. (45) and Ohkawa etal. (46) reported that GAj enhanced the growth and alkaloid production in the hairy root culture of Datura innoxia, the effects of GAj vs lAA, IB A or NAA combinations on the growth and phenolic production were determined. The roots (ca 50 mg, fw) were inoculated in MS liquid media (50 ml / 100 ml flask) containing various combinations of GAj (0,0.001,0.01,0.1 or 1 mg/1) and 1 mg/1 lAA, IBA or NAA and cultured for 4 weeks (Table 3). In the combination with 1 mg/1 IBA or NAA, GAj strongly inhibited the growth of the roots. GAj also exhibited no prominent effect on phenolic production when 1 mg/1 lAA, IBA or NAA was present in the culture medium. [Pg.425]

The hairy roots (ca 70 mg, fw) were inoculated into hormone-free 1/2 MS and B5 liquid media (50 ml / flask) and cultured in the dark on a rotary shaker (100 rpm). The growth is shown in Fig. 5. In both media, the hairy roots grew successfully and reached the maximum levels (3.86 g, fw in 1/2 MS and 2.22 g, fw in B5) at week 6. In these cultures, after 2 to 3 weeks, the tips of the hairy roots began to show a blueish purple colour which was presumed to be caused by a complex between the phenolics in the hairy roots and metal ions contained in the culture medium (Fig. 6). The appearance of this colour also indicated that the hairy roots were rich in phenolics such as tannins. As shown in Fig. 6, the hairy roots grew with rich branching only when they were cultured in 1/2 MS medium. [Pg.430]

Effects of the constituents in the culture medium on phenolic production in the hairy root cultures... [Pg.432]

To determine the effects of the constituents in the culture medium on phenolic (tannin) production in G. thunbergii hairy roots, two culture media were prepared one consisted of 1/2 MS major and minor elements (inorganic elements) and B5 vitamins (named as "1/2 MS vB5") and the other B5 major and minor elements and MS vitamins (B5 vMS). The hairy roots (ca 70 mg, fw) subcultured in 1/2 MS and B5 liquid media, respectively, were inoculated into four hormone-free liquid media (1/2 MS, B5, 1/2 MS vB5 and B5 vMS). After four week-culture in the dark at 25 C, the contents of 1, 2, 5, 6, 12 and 13 in these hairy roots were determined by HPLC (Table 5). [Pg.432]

Although the emetine and the cephaeline could be detected in the first passage calli, they were undetectable by the third passage (section 2.1). However, root culture, especially root culture in liquid medium, showed consistent levels of alkaloids even after the third culture passage. In order to increase the yield of the alkaloids in root culture, culture conditions, such as various ph5dohormones and basal media were investigated [15, 54]. [Pg.689]

Shoot regeneration occurred spontaneously when the roots were cultured on HF 1/2 MS solid medium for over 6 months without exchanging the culture medium, Fig. (56A). The regenerated shoots were excised and transferred onto FIF B5 solid medium containing 3% sucrose (30 ml in a 3... [Pg.723]


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See also in sourсe #XX -- [ Pg.196 ]




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Culture media

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