Datura innoxia

Jackson, P.J., Naranjo, C.M., McClure, P.R. Roth, E.J. (1985). The molecular response of cadmium resistant Datura innoxia cells to heavy metal stress. In Cellular and Molecular Biology of Plant Stress, ed. J.L. Key and T. Kosuge, pp. 145-60. New York Alan R. Liss. 

The (S)-tropic acid, the acid moiety found in hyoscyamine (27) and scopolamine (89), is formed from phenylalanine by an intramolecular 2,3-carbonyl shift (5,124). Feeding of the four possible stereoisomers of [l-14C,3-3H]phenylalanine to Datura innoxia and D. stramonium was used to prove that during the 2,3-carbonyl shift a 3,2-hydrogen shift takes place as well (725)(Scheme 23). With D. innoxia, the 2,3-carbonyl shift was shown to... 

Ke, H.-Y. D. and Rayson, G. D. (1992). Characterization of cadmium binding sites on Datura innoxia using cadmium-113 NMR spectrometry, Environ. Sci. Technol., 26, 1202-1205. 

Dechaux, C. and Boitel-Conti, M. 2005. A strategy for overaccumulation of scopolamine in datura innoxia hairy root cultures. Acta Biologica Cracoviensia Series Botanica, 47(1) 101-107. 

Janardhanan, K. K., and Hussain, A. (1983). Studies on isolation, purification and identification of tenuazonic acid, a phytotoxin produced by Alternaria alternata (Fr.) Keissler causing leaf blight of Datura innoxia Mill. Mycopathologia 83,135-140. 

Datura innoxia Mill. D. met el L. D. stramonium L. China Scopolamine, hyoscyamine, daturodiol, daturolone.33 This herb is mildly toxic. A spasmolytic, analgesic, antiasthmatic, antirheumatic agent. A general anesthetic for major operations. 

C. aurantium, Crataegus laevigata, C. monongyna, C. oxyacantha, Cryptotaenia japonica, Datura innoxia, D. metel, D. stramonium, Eleutherocossus senticosus, Ferula assa-foetida, Fraxinus ornus,... 

Delhaize, E., Robinson, N.J. Jackson, P.J. (1989). Effects of cadmium on gene expression in cadmium-tolerant and cadmium-sensitive Datura innoxia cells. Plant Molecular Biology 12, 487-97. 

Scopolamin may be isolated from seeds of Datura innoxia genus Solanaceae. 

Jousse C, Vu TD, Tran TLM, A1 BaUdii MH, Molinie R, Boitel-Conti M, Pilard S, Mathiron D, Hehn A, Bourgaud F, Gontier E (2010) Tropane alkaloid profiling of hydroponic Datura innoxia Mill, plants inoculated with Agrobacterium rhizogenes. Phytochem Anal 21 118-127... 

Papoutsis I, Nikolaou P, Athanaselis S, Stefanidou M, Pistos C, SpUiopoulou C, Maravelias C (2010) Mass intoxication with Datura innoxia - case series and confirmation by analytical toxicology. Clin Toxicol 48 143-145... 

Tenuazonic Acid. Tenuazonic acid [62] Is a fungal metabolite that has a broad phytotoxicity spectrum (soybean, rice. Datura Innoxia. and lettuce) (235-2371. Toxicity to plants has been attributed to reduced protein synthesis via ribosome Inhibition (211. 21S). 

When the production of the secondary metabolites coincides with the death and general lysis of the cells, the recovery of the product is simply a matter of separation from the spent production solution downstream of the reactor. An example of this type of operation was initially used in Japan during the production of shikonin. However, if the secondary metabolites are stored in the vacuole of the cells and the cells remain viable but dormant during the production phase, then a permeabilizing agent such as dimethylsulfoxide (DMSO), detergents, proteins, and antibiotics may be employed in some cases in concentrations that make the cells leak product out but maintain cell viability. Success for this type of product recovery has been reported in C. roseus, Datura innoxia, and Daucus carota cell cultures. 

Lanoue A, Boitel-Conti M, Portals JC, Laberche JC, Barbotin JN, Christen P, Sangwan-Norreel B. Kinetic study of Uttorine rearrangement in Datura innoxia hairy roots by 13C NMR spectroscopy. J. Nat. Prod. 2002 65 1131-1135. 

The adventitious root cultures of Datura innoxia, Duboisia hybrid M-II-8-14 (a cross-bred between D. myoporoides and D. leichhardtii), and Scopolia tangutica were established from the axenic shoot cultures or intact plants (in the case of Duboisia) on MS solid medium containing 0.1 mg/1 NAA or 1.0 mg/1 lAA. The adventitious roots were maintained in MS liquid medium containing the same phytohormones (0.1 mg/1 NAA or 0.5 mg/1 lAA) in the dark. Addition of auxin in the culture medium has been employed for the maintenance of adventitious root cultures [14], however, the adventitious roots of H. albus and H. niger were induced and maintained in hormone-free 1/2 MS medium [15]. 

Hairy roots of Datura innoxia, Duboisia hybrid M-II-8-6, Scopolia tangutica and the Hyoscyamus species were established by the co-culture method using their leaf discs with A. rhizogenes 15834. In addition, H. albus was transformed with A. rhizogenes strain MAFF 03-01724 [15]. 

Since Kamada etal. (45) and Ohkawa etal. (46) reported that GAj enhanced the growth and alkaloid production in the hairy root culture of Datura innoxia, the effects of GAj vs lAA, IB A or NAA combinations on the growth and phenolic production were determined. The roots (ca 50 mg, fw) were inoculated in MS liquid media (50 ml / 100 ml flask) containing various combinations of GAj (0,0.001,0.01,0.1 or 1 mg/1) and 1 mg/1 lAA, IBA or NAA and cultured for 4 weeks (Table 3). In the combination with 1 mg/1 IBA or NAA, GAj strongly inhibited the growth of the roots. GAj also exhibited no prominent effect on phenolic production when 1 mg/1 lAA, IBA or NAA was present in the culture medium. 

The sequence by which the tropane nucleus as in (26) becomes hydroxylated to give alkaloids like meteloidine (27) and 3a-tigloyloxy-tropane-7)3-ol is unknown. The most recent evidence is conflicting. On the one hand 3a-tigloyloxytropane, doubly labelled as shown in (26), was found to be an intact and specific precursor for meteloidine (27) in Datura innoxia, although both the 3a-tigloyloxytropane (26) and... 

Two non-identified tropane alkaloids have been isolated from aerial parts of Scopolia tangutica. Correlation between total alkaloid content in Belladonna leaves and roots has been made. Regional differences in tropane alkaloid formation of atropine and scopolamine have been studied, using Atropa belladonna. Uptake of Vitavax-6 (10) via the C-labelled species was followed in Datura innoxia, a... 

All selected species showed different behavior towards different dormancy breaking treatments (Figure 1-8). Solarium incanum and S. melongena showed response to maximum number of treatments, whereas response of Datura innoxia recorded only for a few treatments. Solarium erianthum, S. melongena and S. incanum have 100% germination, following pre-chilling and 80%, 100%, 90%, respectively after GA3... 

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