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Culture advising

Implement Pilot Data Collection Exercise in Supportive Culture In order to ensure that the data collection system has been thoroughly checked and tested prior to its laimch, it is advisable to test it in a plant or plant area where there is likely to be a supportive culture. This will allow the effectiveness of the system to be addressed prior to a larger-scale implementation in a less controlled environment. [Pg.290]

A 59-year-old woman presents to an urgent care clinic with a 4-day history of frequent and painful urination. She has had fevers, chills, and flank pain for the last 2 days. Her physician advised her to immediately come to the clinic for evaluation. In the clinic she is febrile (38.5°C [101.3°F]) but otherwise stable and states she is not experiencing any nausea or vomiting. Her urine dipstick test is positive for leukocyte esterase. Urinalysis and urine culture are also ordered. Her past medical history is significant for three urinary tract infections in the past year. Each of these episodes was uncomplicated, treated with trimethoprim-sulfamethoxazole, and promptly resolved. She also has osteoporosis for which she takes a daily calcium supplement. The decision is made to treat her with oral antibiotics for a complicated urinary tract infection with close follow-up. Given her history what would be a reasonable empiric antibiotic choice Depending on the antibiotic choice are there potential drug interactions she should be counseled on ... [Pg.1030]

We thank Professors Hideki Sakurai, Robert West and Zvi Rappoport for helpful discussions and useful advise. We are also grateful to C. Kabuto, T. Yatabe, S. Doi and H. Kamatani for their experimental contributions and to K. Kobayashi, M. Nakano, T. Kudo, M. Souma and M. Nagashima for theoretical calculations. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, Sports, and Culture of Japan. [Pg.150]

It is advisable to maintain a small culture of cells until the viability of the cell bank is validated. This ensures survival of the line should the freezing procedure fail. [Pg.40]

Another problem that is underestimated in animal cell culture is the routine use of antibiotics. Continuous utilization of these compounds is not advised because it favors the development of resistant microbial strains that become difficult to eradicate, requiring the need for potent antibiotics that could be more toxic to the animal cells. Furthermore, the use of these compounds tends to mask contamination at reduced levels. [Pg.30]

The kinetics of apoptosis induction depend on the specific cell type used and the culture environment, so it is advisable to optimize assay parameters for each cell line and also when changing culture conditions during miniaturization of assays. [Pg.116]

The Meyerhoff Scholars Program contains several major components the Summer Bridge program, mentoring, summer research experience, monetary support (including room and board, tuition and fees, and a book allowance), cultural arts activities, academic advising, travel to present research at national scientific conferences, assistance with graduate and professional school placement, and staff support. [Pg.53]

When the hydroxylation reaction is complete, the reaction product must be recovered from the fermentation culture. The product is dissolved or suspended in the culture medium and can be isolated from the whole broth or from the supernatant after removal of the biomass by filtration, if necessary with a filter aid, or by centrifugation1. Thorough washing of the separated biomass with a buffer or water is advisable as a significant amount of the product might be contained in the solid phase. [Pg.364]

Serum, ascites, or hybridoma culture supernatant can, after filtration, be applied to the column directly. The crude antibody solution should then be diluted with 1/10 volume of IMTris-HCl, pH 8.0, and chromatography should be performed with 100 xaM Tris-HCl, pH 8.0. It is, however, advisable first to precipitate the immunoglobulins with ammonium sulfate see Chapter 2). This not only reduces the sample volume, but more importantly removes lipids, particularly from serum and ascitic fluid, extending the life of the column. [Pg.40]

In an organization that has not historically evaluated performance using objective data, the initial reported results may appear to indicate that performance has declined. This sometimes occurs when the historical perspective is too optimistic. For example, the number of near misses often increases as reliability of reporting improves. The recipients for metrics reports should be advised what to expect and provided explanation as to why the results may not meet expectations. The potential cultural impact, if left unaddressed, may raise doubt about the accuracy and value of the metrics reports, and these potential negative reactions need to be managed. [Pg.108]


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See also in sourсe #XX -- [ Pg.143 , Pg.145 ]




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Advisers

Advising

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