Crosslinking recombination

Chang TM. Modified hemoglobin-based blood substitutes crosslinked, recombinant and encapsulated hemoglobin. Vox Sang 74(suppl 2) 233, 1998. 

When discussing crosslinking, recombination is the only useful process. Disproportionation leads to a formation of unsaturation (double... 

By using an in vitro UV-crosslinking approach, bacteria-purified recombinant CPEB3 has been demonstrated to interact with the 2> UTR of GluR2 mRNA. To test whether this interaction occurs in vivo, hippocampal neuron cultures are ultraviolet (UV)-irradiated, homogenized, and immunoprecipitated with CPEB3 IgG or nonspecific IgG (Huang et al, 2006). Three plates of 2- to 3-week-old cultured neurons ( 6—8 million... 

Ghetie, V., Till, M.A., Ghetie, M.-A., Tucker, T., Porter, J., Patzer, E.J., Richardson, J.A., Uhr, J.W., and Vitetta, E.S. (1990) Preparation and characterization of conjugates of recombinant CD4 and deglyco-sylated ricin A chain using different crosslinkers. Bioconjugate Chem. 1, 24-31. 

Only a limited number of peroxides are suitable for crosslinking purposes. The most suitable are those which form radicals through homolytic decomposition. These radicals are so aggressive that they can split hydrogen atoms from the polymer chain, producing stable peroxide decomposition products and polymer radicals. These polymer radicals can then be recombined, producing stable C-C crosslink bonds. 

In linear EP of bifunctional monomers, such as S, with water soluble initiators, the monomer droplets do not compete with micelles in capturing radicals from the aqueous phase because the total surface area of the droplets is much smaller than that of micelles and growing particles. Nevertheless, if some radicals enter monomer droplets, rapid termination takes place. Therefore, polymerization in monomer droplets is negligible [88]. However, if in the crosslinking EP of 1,4-DVB a few radicals are captured by monomer droplets, they can polymerize completely because the recombination of radicals is suppressed by the gel effect. Moreover, in thermal initiation or in initiation by hydrophobic initiators, such as AIBN, radicals are formed predominantly in the hydrophobic phase, i.e. in monomer droplets and in micelles, and crosslinking EP is initiated in the organic phase. 

Although mononitrenes do not act as crosslinking agents themselves, they can generate unpaired electrons in the polymer chains by the abstraction of hydrogen molecules from the polymer. As the result, the polymer chains are crosslinked by the recombinations of these unpaired electrons. Crosslinking by mononitrene in such a way, depends on the activity of the nitrene and may be not necessarily less efficient in some combination of monoazide with the polymer than that by dinitrene as is seen in the case for... 

Actin-binding proteins ABP-50, purification, 196, 78 ABP-120, purification, 196, 79 ABP-240 purification, 196, 76 effect on actin depolymerization, 215, 74 extraction, 196, 311 isolation from Dictyostelium discoideum, 196, 70 platelet-derived actin binding proteins [characterization, 215, 58 purification, 215, 58, 64 recombination with actin, 215, 73] 30-kDa Dictyostelium discoideum actin-crosslinking protein [assays, 196, 91 preparation, 196, 84] actin-depolymerizing factor [assay, 196, 132[ DNase assay, 196, 136 platelet-derived a-actinin [characterization, 215, 58 purification, 215, 58, 70 recombination with actin, 215, 73]. 

Importantly, the ERCCl/XPF structure-specific nuclease has an additional role in the repair of cisplatin adducts besides its function in NER the recombinational repair of interstrand crosslinks (19). ERCCl- or XPF-deficient hamster mutant cell lines are hypersensitive to DNA crosslink agents, much more so than to ultraviolet-induced pyrimide dimers, the classical substrates for NER (20,21). Moreover, co-localization of ERCCl foci and RAD51 foci in response to cisplatin treatment has recently been found and may represent recruitment of ERCCl/XPF to sites of recombination repair (22). Previous studies have shown that BRCAl, involved in homologous recombination repair, also plays a major role in the repair of cisplatin DNA damage (23). 

This would lead to the formation of two polymeric radicals in close vicinity, and these would almost certainly recombine to form a crosslink ... 

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