Collagenase, mammalian

Metallo- protease collagenase (mammalian) cleaves a Gly-Leu or Gly-Ile bond in native triple helical collagen... 

Synthetic Inhibitors of Bacterial and Mammalian Interstitial Collagenases... 

BACKGROUND Structure and function of collagens Bacterial and mammalian collagenases Bacterial collagenases... 

Mammalian collagenases and the matrix metalloproteinases Collagenase assays Collagen-based assays Peptide-based assays... 

INHIBITORS OF MAMMALIAN COLLAGENASES Non-substrate analogue inhibitors Substrate analogue inhibitors Phosphorus-based inhibitors Sulphur-based inhibitors Hydroxamate inhibitors tt-Carboxyalkyl inhibitors Miscellaneous inhibitors... 

Table 8.8. PHOSPHONAMIDATE AND PHOSPHORAMIDATE INHIBITORS OF MAMMALIAN COLLAGENASES...

Table 8.13. BIDENTATE a-SUBSTITUTED IN-REGISTER MERCAPTAN INHIBITORS OE MAMMALIAN COLLAGENASES... 

Bacterial and mammalian collagenases their inhibition, 29(1992) 271 1-Benzazepines, medicinal chemistry of, 27... 

CytoTherapeutics filed two patent applications covering the use of NSCs. WO-09911758 disclosed the isolation, characterization, proliferation, differentiation and transplantation of mammalian NSCs, and WO-00050572 covers a method for the in vitro proliferation of NSC cultures using a growth factor and a collagenase enzyme said to increase cell viability and the number of proliferative cells with time. 

Some years ago, collagenase from the microorganisms Clostridium histolyticum was shown to be a zinc metalloenzyme 1303 mammalian collagenases are also zinc metalloenzymes.1304 The collagenase-like peptidase of rat testis1305 has also been shown independently to be a zinc metalloenzyme. 

Fields, GB. (1991). A model for interstitial collagen catabolism by mammalian collagenases. /. Them. Biol. 153, 585-602. 

The specific cleavage of collagen by mammalian collagenases produces two fragments that are unstable at body temperature and begin to uncoil into individual chains. These are then susceptible to attack by other proteolytic enzymes. 

Greenwald RA, Golub LM, Ramamurthy NS, Chowdhury M, Moak SA, Sorsa T. In vitro sensitivity of the three mammalian collagenases to tetracycline inhibition relationship to bone and cartilage degradation. Bone 1998 22(l) 33-8. 

Metalloprotease inhibitors - also known as metalloproteases or zinc proteases - are proteolytic enzymes of which the activity depends on metal ions, normally bound Zn -. Examples of metalloproteases are the pancreatic enzymes carboxypeptidase A and B, elastase, the well-characterized bacterial enzyme thermolysin and the collagenase family (found in both bacterial and mammalian cells, fibroblast collagenase, neutrophil elastase, gelatinase). 

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