Clostridium Purification

Drake HL. S-1 Hu, HG Wood (1980) Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermoaceticum. J Biol Chem 255 7174-7180. 

White H, R Feicht, C Huber, F Lottspeich, H Simon (1991) Purification and some properties of the tungsten-containing carboxylic acid reductase from Clostridium formicoaceticum. Biol Chem Hoppe-Seyler 372 999-1005. 

Yamamoto I, T Saiki, S-M Liu, LG Ljungdahl (1983) Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein. J Biol Chem 258 1826-1832. 

Okeke BC, YC Chang, M Hatsu, T Suzuki, K Takamizawa (2001) Purification, cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethene (PCE) from Clostridium bifermen-tans DPH-1. Can J Microbiol AT. 448-456. 

HeZ,JWiegel (1996) Purification and characterization of an oxygen-sensitive, reversible3,4-dihydroxybenzo ate decarboxylase from Clostridium hydroxybenzoicum. J Bacteriol 178 3539-3543. 

Wagner R, R Cammack, JR Andreesen (1984) Purification and characterization of xanthine dehydrogenase from Clostridium acidiurici grown in the presence of selenium. Biochim Biophys Acta 791 63-74. 

The highest degree of purification was achieved with extracellular endopectate lyase from the anaerobic bacterium Clostridium fel-sineum.Mi A 225-fold purified preparation, homogeneous in disc electrophoresis, was obtained by precipitation with ethanol, and chromatography on CM-cellulose and on Sephadex G-200. Its molecular weight (determined by gel chromatography) was 105,000. 

A purification procedure including all of the separation principles then known led to a 156-fold purification of exopectate lyase of Clostridium multifermentans, but pectinesterase could not be re-moved.51,105... 

Tso, M.-Y., T. Ljones and R.H. Burris. Purification of the nitrogenase protein from Clostridium pasteurianum. Biochim. Biophys. Acta 267,600-604 (1972). 

Bradshaw WH, Barker HA. 1960. Purification and properties of xanthine dehydrogenase from Clostridium cylindrosporum. J Biol Chem 235 3620-9. 

Seto B, Stadtman TC. 1976. Purification and properties of proline reductase from Clostridium sticklandii. J Biol Chem 251 2435-9. 

Stadtman TC, Elliott P. 1957. Studies on the Enzymatic Reduction of amino acids II. Purification and properties of ao-proline reductase and a prohne racemase from Clostridium sticklandii. 1 Biol Chem 228 983-97. 

Das A, Ivey DM, Ljungdahl LG. 1997. Purification and reconstitution into prote-ohposomes of the FiFq ATP synthase from obligately anaerobic bacterium Clostridium thermoautotrophicum. J Bacteriol 179 1714—20. 

Ivey DM, Ljungdahl LG. 1986. Purification and characterization of the Fi-ATPase from Clostridium thermoaceticum. J Bacteriol 165 252-7. 

Lehmann Y, Meile L, Teuber M. 1996. Rubrerythrin from Clostridium perfringens cloning of the gene, purification of the protein, and characterization of its superoxide dismutase function. J Bacteriol 178 7152-8. 

Selected entries from Methods in Enzymology [vol, page(s)] Determination of FMN and FAD by fluorescence titration with apoflavodoxin, 66, 217 purification of flavin-adenine dinucleotide and coenzyme A on p-acetoxymercurianiline-agarose, 66, 221 a convenient biosynthetic method for the preparation of radioactive flavin nucleotides using Clostridium kluyveri, 66, 227 isolation, chemical synthesis, and properties of roseoflavin, 66, 235 isolation, synthesis, and properties of 8-hydroxyflavins, 66, 241 structure, properties and determination of covalently bound flavins, 66, 253 a two-step chemical synthesis of lumiflavin, 66, 265 syntheses of 5-deazaflavins, 66, 267 preparation, characterization, and coenzymic properties of 5-carba-5-deaza and 1-... 

Many examples of the purification of xylanase enzymes to homogeneity can be found in the reviews of Dekker and Richards (85), Woodward (86) and Reilly (87). Other xylanases which have been prepared recently to very high purity using traditional biochemical techniques include xylanases from Sporotrichum dimorphosporum (88) Streptomyces sp. (71) Trichoderma harzianum (5,55) Clostridium acetobuiylicum (30,89) mesophilic fungal strain Y-94 (80) Aspergillus nigcr (90-92) and several thermostable xylanases discussed above. 

The formation of 1,3-propanediol from glycerol by Klebsiella pneumoniae, Citrobacter and Clostridium butyricum, respectively as well as 2,3-butanediol by Enterobacter aerogenes and their recovery and purification were central issues as well. The production was partly performed in a 2000 litre reactor. Glycerol metabolism in these microorganisms was established. In addition the application of the diols was investigated. 

Schaupp, A. Ljungdahl, L.G. Purification and properties of acetate kinase from Clostridium thermoaceticum. Arch. Microbiol., 100, 121-129 (1974)... 

Table 3 shows the recovery and extent of enrichment in each of the various purification steps of ferredoxin from Clostridium acidi-urici by using DEAE-cellulose and ammonium sulfate fractionation (Buchanan, Lovenberg, and Rabinowitz (32)). This procedure yields crystalline preparations from all clostridia which have been tested. Various modifications are employed for the crystallization of ferredoxin from other bacteria (Figs. 1 and 2). 

Table 3. Purification and crystallization of ferredoxin from Clostridium acidi-urici ...

Debeire, P., Priem, B., Strecker, G., and Vignon, M., Purification and properties of an endo-l,4-xylanase excreted by a hydrolytic thermophilic anaerobe, Clostridium thermolacticum. A proposal for its action mechanism on larchwood4-0-methylglucuronoxylan. Eur J Biochem 1990, 187 (3), 573-80. 

Mortenson, L.E., Morris, J.A., and Jeng, D.Y (1967) Purification, metal composition, and properties of mollibdoferredoxin and azoferredoxin, two components of thenitrogen-fixing system of Clostridium pasterianum, Biochim. Biophys. Acta 141, 516-538. 

Holloway, D. E., and Marsh, E. N. G., 1994, Adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum. Overexpression in Escherichia coli, purification, and characterization of the recombinant enzyme. J. Biol. Chem. 269 20425920430. 

Leutbecher, U., Bocher, R., Linder, D., and Buckel, W., 1992, Glutamate mutase from Clostridium cochlearium. Purification, cobamide content and stereospecific inhibitors, Eur. J. Biochem. 205 7599765. 

Jung /V, Just I, van Damme J et al. (1993) NAD-binding site of the C3-like ADP-ribosyltransferase from Clostridium limosum. In J. Biol. Chem. 268 23215-8 Just I, Mohr C, Schallehn G et al. (1992a) Purification and characterization of an ADP-ribosyltransferase produced by Clostridium limosum. In J. Biol. Chem. 267 10274-80... 

Laudanna C, Campbell JJ, Butcher EC (1996) Role of Rho in chemoattractant-activated leukocyte adhesion through integrins. In Science 271 981 -3 Machesky LM, Hall A (1996) Rho a connection between membrane receptor signalling and the cytoskeleton. In Trends Cell Biol. 6 304—10 Maehama T, Ohoka Y, Ohtsuka T et al. (1990) Botulinum ADP-ribosyltransferase activity as affected by detergents and phospholipids. In FEBS Lett 263 376-80 Malcolm KC, Ross AH, Qiu R-G etal. (1994) Activation of rat liver phospholipase D by the small GTP-binding protein RhoA. In J. Biol. Chem. 269 25951 -4 Moriishi K, Syuto B, Yokosawa N efal. (1991) Purification and characterization of ADP-ribosyltransferases (Exoenzyme C3) of Clostridium Botulinum type C and D strains. In J. Bacferiol. 173 6025-9... 

Just I, Mohr C, Schallehn G et al. (1992) Purification and characterization of an ADP-ribosyltransferase produced by Clostridium limosum. J. Biol. Chem. 267 10274-10280. 

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