1,3-propanediol from glycerol

The formation of 1,3-propanediol from glycerol by Klebsiella pneumoniae, Citrobacter and Clostridium butyricum, respectively as well as 2,3-butanediol by Enterobacter aerogenes and their recovery and purification were central issues as well. The production was partly performed in a 2000 litre reactor. Glycerol metabolism in these microorganisms was established. In addition the application of the diols was investigated. 

R., and Zeng, A.-P. (2014) Cell-free synthesis of 1,3-propanediol from glycerol with a high yield. Eng. Life Set, 14, 380-386. 

Li and coworkers (2001a,b,c, 2002) used silicate-1, ZSM-5, ZSM-11, mordenite, and X-, Y-, and p-type zeolite membranes to remove 1-3-propanediol from glycerol... 

Li, S., Tuan, V.A., Falconer, J.L. and Noble, R.D. 2001c. Effects of zeolite membrane structure on the separation of 1,3-propanediol from glycerol and glucose by pervaporation. 

K., Celinska, E., Spychala, M., Szymanowska-Powalowska, D., Czaezyk, K., and Grajek, W. (2012) Physiological predisposition of various Clostridium species to synthetize 1,3-propanediol from glycerol. Process Biochem., 47, 1308-1319. 

Anand P, Saxena RK, Marwah RG. (2011). A novel downstream process for 1,3-propanediol from glycerol-based fermentation. Appl Microbiol Biotechnol, 90,1267-1276. 

Gao SJ, Zhang DJ, Sun YQ, XiuZL. (2007). Separation of 1,3-propanediol from glycerol-based fermentations of Klebsiella pneumoniae by alcohol precipitation and dilution crystallization. Front Chem Eng China, 1, 202-207. 

Gonzalez-Pajuelo M, Meynial-Salles I, Mendes F, Andrade JC, Vasconcelos I, Soucaille P. (2005b). Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metab Eng, 1, 329-336. 

Huang YN, Li ZM, Shimizu K, Ye Q. (2012). Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae. Bioresource Technol, 103, 351-359. 

Li SG, Tuan VA, Falconer JL, Noble RD. (2001c). Separation of 1,3-propanediol from glycerol and glucose using a ZSM-5 zeolite membrane. J Membrane Sci, 191, 53-59. 

Li ZG, Jiang B, Zhang DJ, Xiu ZL. (2009). Aqueous two-phase extraction of 1, 3-propanediol from glycerol-based fermentation broths. Sep PurifTechnol, 44, 112-117. 

Li ZG, Teng H, Xiu ZL. (2011). Extraction of 1,3-propanediol from glycerol-based fermentation broths with methanoEphosphate aqueous two-phase system. Process Biochem, 46, 586-591. 

Liu HJ, Zhang DJ, Xu YH, Mu Y, Sun YQ, Xiu ZL. (2007). Microbial production of 1,3-propanediol from glycerol by Klebsiella pneumoniae under micro-aerobic conditions up to a pilot scale. Biotechnol Lett, 29, 1281-1285. 

Petrov K, Stoyanov A. (2012). Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations. Bioproc Biosyst Eng, 35, 317-321. 

Ashok S, Raj SM, Rathnasingh C, Park S. (2011). Development of recombinant Klebsiella pneumoniae AdhaT strain for the co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol. Appl Microbiol Biotechrwl, 90,1253-1265. 

Figure 1.7 Metabolic pathway for the production of 1,3-propanediol from glycerol.

Kumar V, Sankaranarayanan M, Jae K-e, et al. Co-producfron of 3-hydroxypropionic acid and 1, 3-propanediol from glycerol using resting cells of recombinant Klebsiella pneumoniae J2B strain oveiex-pressing aldehyde dehydrogenase. Appl Microbiol Biotechnol 2012 96 373-83. 

Klebsiellia pneumoniae 1,3-Propanediol Using EMA to investigate the metabolic capabilities of K. pneumonia for producing 1,3-propanediol from glycerol under aerobic and anaerobic conditions. No Zhang and Xiu (2009) s... 

Gunzel B, Yonsel S, Deckwer WD (1991) Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum up to a scale of 2 m. Appl Microbiol Biotechnol 36 289-294 Gutierrez NA, Maddox IS (1992) Product inhibition in a nonmotile mutant of Clostridium acetobutylicum. Enz Microbiol Technol 14 101-105 Hamilton GA, Westheimer FH (1959) A crystalline decarboxylase without biotin. J Am Chem Soc 8 2277... 

Moreira AR, Ulmer DC, Linden JC (1981) Butanol toxicity in butylic fermentation. Biotechnol Bioengin Symp 11 567-579 Nagarajan V, Nakamura CE (1998) Production of 1,3-propanediol from glycerol by recombinant bacteria expressing recombinant diol dehydratase. US Patent 5821092... 

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