Enzymes xylanase

Tarvainen, K., Kanerva, L., Tupasela, O., Grenquist-Norden, B., Jolanki, R., Estlander, T., and Keskinen, H. (1991). Allergy from cellulose and xylanase enzymes. Clin. Exp. Allergy 21, 609-615. 

Many examples of the purification of xylanase enzymes to homogeneity can be found in the reviews of Dekker and Richards (85), Woodward (86) and Reilly (87). Other xylanases which have been prepared recently to very high purity using traditional biochemical techniques include xylanases from Sporotrichum dimorphosporum (88) Streptomyces sp. (71) Trichoderma harzianum (5,55) Clostridium acetobuiylicum (30,89) mesophilic fungal strain Y-94 (80) Aspergillus nigcr (90-92) and several thermostable xylanases discussed above. 

Figure 2. Heat inactivation profile of the xylanase in the culture filtrate from Thermoascus aurantiacus. Xylanase enzyme was prepared as described by Tan et al. (74). Xylanase and endocellulase activities were determined after the enzyme was incubated for the specified time at 70°C. Aliquots were removed and assayed at 50°C by the method described by Yu et al. (75). Activities were expressed as a percentage of the control stored at 4°C.

Although wheat middlings contain a substantial amount of NSP, Im et al. (1999) were able to demonstrate only a slight improvement in the nutritive value of middlings for broilers following xylanase supplementation of the diet. On the other hand, Jaroni et al. (1999) were able to demonstrate an improvement in egg production in Leghorn layers by supplementation of a middlings-based diet with protease and xylanase enzymes. 

Enzyme formulation based on cellulase and xylanase enzymes for poultry feed ... 

Sigoillot, C., Lomascolo, A., Record, E., Robert, J. L., Asther, M., Sigoillot, J. C. (2002). Lignoeellulolytic and hemicellulolytic system of Pycnoporus cinnabarinus isolation and characterization of a cellobiose dehydrogenase and a new xylanase. Enzyme and Microbial Technology, 31, 876-883. 

Table 1 Effects of cellobiose and cello-oligosaccharides supplements on growth and production of extracellular cellulase and xylanase (enzyme activities per gram mycelia mass).

Csiszar, E., Urbanszki, K., and Szakacs, G. (2001). Biotreatment of desized cotton fabric by commercial cellulase and xylanase enzymes, I. Mol. 

By preparing chimeric xylanase, enzymes with increased catalytic activity and alters pH optimum were successfully obtained as described below. 

Binding of xylanase components shake flasks by SF, has been carried out on sulfite pulps (8). Although xylanases from A. oryzae has been produced exclusively in SF (4,8,14,31), a SSF alternative has been demonstrated most recently (IS). However, no reports are found in flie iit ture on the use of xylanase enzymes produced by this fringus in SSF for biobleaching of cellulose pulps. 

Enzeco Xylanase [Enzyme Development http //www. enzymedeveiopment. com] Lyxasan t[BASF AG http //www.basf.de]... 

DIhydroxyacetone Phytase Xylanase enzyme activator, barley malting Potassium gibberellate enzyme activator, brewing Gibberellic acid... 

Although efficient and novel genetic methods have been employed by various researchers to overexpress a few components of cellulase and xylanase enzymes in several hosts, information on modification of the properties of the recombinant enzymes is rather scanty (cf. section 5.3). Furthermore, the enzyme components which have been overexpressed are unlikely to hydrolyze cellulose efficiently unless mixed with other components to have a complete enzyme system, which is required for efficient hydrolysis. This is perhaps the reason as to why such recombinant organisms have not been widely studied for production of the enzymes in bench or pilot-scale bioreactors, which is required to assess the economics of their large-scale production. The production of hydrolytic enzymes economically is of prime concern for their subsequent commercial application in bioproduct manufacture. For successful industrial... 

The role of cellulase and xylanase enzymes is crucial in realizing the aim of producing ethanol (and other biofuels) and commodity bioproducts from cellulosic feedstocks. The cost of producing cellulase has been brought down significantly over the last... 

Chivero ET, Mutukumira AN, Zvauyu R. (2001). Partial purification and characterization of a xylanase enzyme produced by a microorganism isolated from selected indigenous fruits of Zimbabwe. Food Chem, 72, 179-185. 

N. D. Yilmaz, E. ah kan, K. Yilmaz, Effect of xylanase enzyme on mechanical properties of fibres extracted from undried and dried corn husks. Indian. Fibre Text. Res. 39, 60-64 (2014). 

Tarvainen K, Kanerva L, Tupasela B, Grenquist-Norden B, Jolanki R, Estlander T, Keskinen H (1991b) Allergy from cellulase and xylanase enzymes. Clin Exp Allergy 21 609-615 Tiikkainen U, Louhelainen K, Nordman H (1996) Flour dust. Arbete och Halsa 27... 

Monilia sp, isolated from bagasse compost was found to utilize many polysaccharides including cellulose and produce ethanol, and cellulase and xylanase enzymes [61]. Cellulase activity was detectable in the culture medium after 48 h of growth. Interestingly, when cellulose was used as a substrate, both cellulase and... 

All the strains were found to utilize cellulose and produce extracellular cellulase and xylanase enzymes. F. oxysporum 841 was found to be a potential mould for direct conversion of cellulose to ethanol/acetic acid. Though other species, e.g. F. oxysporum 2018, 62287 and 62291 also produced comparable amounts of cellulase and xylanase enzymes, only traces of ethanol and acetic acid were detected in non-aerated cultures. Maximiun cellulase activity was observed after 96 h of bioconversion process. F. oxysporum VTT-D-80134, however, was not found to produce sufficient cellulolytic and xylanolytic activities to convert cellulose or hemicellulose directly into ethanol [81]. 

The composition of multienzyme preparation PC-PEB-9 and enzyme preparation PC-HOST based on P. canescens PCXlnR host strain was determined using Fast Protein Liquid Chromatography (FPLQ and data of enzymatic activities of BGL, PEL, xylanase, and EG2 toward PNPG, citrus pectin, birch wood xylan, and CMC, respectively (Table 3). Enzyme preparatirMi PC-HOST contained 30 % of xylanases. Enzyme preparation PC-PEB-9 consisted of 15 % xylanase, 11 % PEL, 18 % EG2, and 12 % BGL (Bushina et al. 2012). 

Lee, C.C., Kibblewhite-Acdnelli, R.E., Wagschal, K., Robertson, G.H., Wong, D.W.S., 2006. Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library. Extremophiles 10 (4), 295-300. 

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