Clostridium acidi-urici

Barker HA, Beck JV. 1941. The fermentative decomposition of pnrines by Clostridium acidi-urici and Clostridium cylindrosporum. J Biol Chem 141 3-27. 

Table 3 shows the recovery and extent of enrichment in each of the various purification steps of ferredoxin from Clostridium acidi-urici by using DEAE-cellulose and ammonium sulfate fractionation (Buchanan, Lovenberg, and Rabinowitz (32)). This procedure yields crystalline preparations from all clostridia which have been tested. Various modifications are employed for the crystallization of ferredoxin from other bacteria (Figs. 1 and 2). 

Table 3. Purification and crystallization of ferredoxin from Clostridium acidi-urici ...

Raeburn, S. and J. C. Rabinowitz Pyruvate synthesis by a partially purified enzyme from Clostridium acidi-urici. Biochem. Biophys. Res. Commun. 18, 303-307 (1965). 

Pyruvate Ferredoxin oxido-reductase from Clostridium acidi-urici. In... 

H.A. Barker, S. Ruben and J.V. Beck, Radioactive Carbon as an Indicator of Carbon Dioxide Reduction. IV. The Synthesis of Acetic Acid from Carbon Dioxide by Clostridium Acidi-Urici, Proceedings of the National Academy of Sciences 26, 477-482, 1940. 

Figure 10. Crystals of Clostridium acidi-urici ferredoxin and some derivatives...

Figure 13. Precipitation reaction by antiserum to Clostridium acidi-urici ferredoxin...

Wilson (1967) has reported on the use of low temperatures (— 196 C) to sharpen the absorption bands of some nonheme iron proteins, e.g., ferredoxins of spinach and of Clostridium acidi-urici, and an adrenal protein. All three of these nonheme proteins have absorption maxima near 700 nm which disappear on reduction by dithionite. The oxidized spinach ferredoxin has two additional absorptions, a shoulder near 830 nm and a weak maximum at 930 nm. Wilson (1967) investigated the possibility that the absorbance of these bands, particularly the 714 nm band of spinach ferre-doxin, is sensitive to protein conformational changes in a manner analogous to the 695 nm band of ferricytochrome c (Schejter and George, 1964). 

Purine Degradation by Anaerobic Bacteria. A totally different degradation of purines carried out by certain anaerobic bacteria, Clostridium acidi-urici and C. cylindrosporum has been largely elucidated by Rabinowitz. 

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