2- cleaved

Cleave the peptide into smaller fragments separate these fragments and determine the ammo acid composition of the fragments... 

Phenacyl esters can be prepared from the phenacyl bromide, a carboxylic acid, and potassium fluoride as base. These phenacyl esters can be cleaved by irradiation (313 nm, dioxane or EtOH, 20°, 6 h, 80-95 % yield, R = amino acids > 300 nm, 30°, 8 h, R = a gibberellic acid, 36-62% yield ). Another phenacyl derivative, RC02CH(C0C6H5)C6H3 3,5-(0CH3)2, cleaved by irradiation, has also been reported. ... 

Table 2 depicts an approach to the modification of biomass with a view to effecting necessary changes, structural or otherwise, in natural polymers. Conventionally, modifications [54-63] are effected mainly by two ways (1) mechanical and/or chemical modifications without destroying the main structural backbone, and (2) cleav-... 

H2O2 The chemical symbol for hydrogen peroxide Hag Haemagglutinin Hag-1, Hag-2 Cleaved haemagglutinin subunits-1, -2 H E Haematoxylin and eosin hIL Human interleukin Hb Haemoglobin... 

FIGURE 4 Electropherogram of somatropin CRS (A) Electropherogram of the EP reference solution somatropin CRS I.Omg/ml (B) blank. The major peak corresponds to somatropin. The peaks 1—5 correspond to the impurities described by the Ph.Eur. (2) cleaved form (3) Gin-18 somatropin (4) deamidated forms. Temperature 30 C voltage program 0—l7.4kV. For more conditions see text. 

After DNA binds (step (T)), an active-site Tyr attacks a phosphodiester bond on one DNA strand in step (2), cleaving it, creating a covalent 5 - —Tyr protein-DNA linkage, and liberating the 3 -hydroxyl group... 

Cleave both strands of the DNA carrier with a restriction endonuclease. 

Selective cleavage of methoxythioanisoles. o-, rn-, and p-Methoxythioanisoles are selectively cleaved by sodium in HMPT to methoxythiophenols by cleavage of the CH3—S bond. Cleavage of the C—O bond occurs to a minor extent (less than 5%) in the case of o- and m-methoxythioanisoles. In contrast, sodium isopropanethiolate, (CH3)2CHSNa,2 cleaves the same substrates selectively to the sodium salts of (methylthio)phenols. Again, this process is not completely selective in the case of o-and m-methoxythioanisoles. Sodium isopropanethiolate also effects dealkylation of uryl alkyl sulfides when HMPT is used as solvent, and this reaction provides a convenient synthesis of aromatic thiols.3... 

Lin, X., Koelsch, G, Wu, S., Downs, D., Dashti, A., Tang, J. Human aspartic protease memapsin 2 cleaves the (3-secretase site of (3-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 2000, 97, 1456-1460. 

Step 2 cleaves the silyl protecting group using a standard protocol with tetrabutylammonium fluoride (TBAF) in THF. 

Cleaving reagent trifluoroacetic acid (TFA Shanghai Fluoride Chemicals, Shanghai, China). 

Cleave the Dde group from the resin with 2% hydrazine and 1% allyl alcohol in DMF for 3 x 3 min (see Note 5). Wash the resin with DMF three times. Reagent needed hydrazine. 

The source of organic materials. Possible sources cited have included mineral-catalyzed hydrothermal synthesis, atmospheric syntheses driven by radiation or electrical discharges (see Section 5.1), and delivery from outer space (see Section 5.2). Cleaves and Chalmers have summarized the alternatives.61... 

Cleave several -fig aliquots of the DNA with different restriction endonucleases, following the manufacturer s recommendations. Fractionate the digested DNA by electrophoresis through 0.8% (w/v) agarose gels, then visualize the DNA by ethidium bromide staining and transillumination. 

Step [2] Cleave the three n bonds and use arrows to show where the new bonds will be formed. 

Other reactions include the apparent oxidative addition of the OH radical from water to [t -(Me3Si)2C5H3]2Ln (Ln = Sm, Yb) to give [)j -(Me3Si)2C5H3]2Ln( i-OH) 2 complexes, which have been structurally characterized . The complex (f] -Cp )2Sm(THF)2 cleaves the C-N bond of isonitriles to give the cyano complex, ( -Cp )2Sm(CN) (CNR), among other products . 

NH2NH2,H2O,MeOH,0°Ctort, l-2h Hg(OAc>2, 2-3horCu(OAc>2, 3-24b HSCH2CH2OH, 71-92% yield. These conditions return the free tbiol. Tbe use of Hg(OAc)2 cleaves tbe Acm (acetamidometbyl) group in the presence of the Pirn group. 

The configurations at C(4 ) of the hexahydroquinines were established62 by conversions1" (see also p. 213) to the corresponding N(l )—C(2 ) cleaved quinoli-zines in which the stereochemistry could be assigned by n.m.r. 

The reactive intermediate Fe(dmpe)2 cleaves the C—H bonds of other aromatic substrates, but the reactions are complex. The final products with anisole or methyl benzoate are methyl derivatives derived from cleavage of their respective O—CH3 bonds. Chlorobenzene gives a transient aryl hydride, but the ultimate product is the phenyliron chloro complex. Both Ru(dmpe)2H(CioH7) and Os(dmpe)2H(CioH7) are also in equilibrium with their respective M(dmpe)2 intermediates, but the equilibria are very sluggish and of little synthetic utility. An alternative approach to these species is photochemical activation of Fe(dmpe)H2 the products can be obtained more cleanly, and the intermediate Fe(dmpe)2 cleaves even aliphatic C—H bonds. 

EFEs have relatively broad substrate specificities, such as trypsin (cleavage site Lys-X and Arg-X, X represents an amino acid) and chymotrypsin (cleavage site Phe-X, Trp-X, Tyr-X and Leu-X), as shown in Fig. (9) [28]. F-III-2 cleaves [3-amyloid 1-40 at six sites, and oxidized insulin B-chain at five sites and F-II digested them at six and ten, respectively [28]. 

Uranyl Acetate. The complex U02(OAc)2 cleaves HIV mRNA upon photoactivation in a uniform fashion unless the nucleic acid is associated with protein (94). Recently, it has been shown that intense, specific cleavage occurs on RNA which is bound to HeLa cell nuclear protein. Rather an imprint than a footprint, this strong cleavage indicates that the structure of the RNA and its accessibility to metal complexes in solution is changed in the presence of protein. 

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