Detection of Caspases, Cleaved Substrates, Regulators, and Inhibitors

5c Detection of Caspases, Cleaved Substrates, Regulators, and Inhibitors. There are more than 13 known caspases (procaspases or active cysteine caspases) that can be detected using various types of caspase activity assays. There are also immunohistochemistry assays that can detect cleaved substrates such as 

Apoptosis PCR microarray is a relatively new methodology that uses real-time PCR to profile the expression of at least 112 key genes involved in apoptosis. The array includes the TNF ligands and their receptors members of the bcl-2, caspase, IAP, TRAF (TNF receptor associated factor), CARD (caspase activation and recruitment domain), death domain, death effector domain, and CIDE (cell death-inducing DFFA-like effector) families and genes involved in the p53 and ATM pathways. Also included in the evaluation are genes involved in anti-apoptosis. This assay allows for the evaluation of the expression of a focused panel of genes related to apoptosis. 

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Detection of caspases, cleaved substrates, regulators and inhibitors... 

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