Substituent-cleaving enzymes hydrolysis

First attempts to predict the selectivity of enzymes are dated back to 1964 when Prelog described an empirically determined rule for the addition of hydrogen to ketones by the yeast Culvaria lunata [13]. In 1991 Kazlauskas published the hydrolysis of acetates of secondary alcohols by Pancreatic cholesterol esterase, Pseudomonas cepacia and Candida rugosa and formulated the widely applicable Kazlauskas rule according to which esters of secondary alcohols with a specific substitution pattern of large (L) and medium (M) substituents are cleaved faster than the corresponding enantiomer [14]. 

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