Selective chemical methods for cleaving peptide bonds

8 Selective chemical methods for cleaving peptide bonds 

In order to obtain fragments from a protein of a suitable size for sequencing by the Edman method, it is desirable to effect specific cleavage adjacent to the rarer amino acids. Numerous chemical methods of specifically cleaving proteins have been devised, but only a few are in common use. 

Several other methods for the selective chemical cleavage of peptide bonds adjacent to particular amino acids have been described, but none has been used widely. 

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