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Proteomics analysis cleave proteins

Other proteolytic enzymes commonly used in proteomics analysis include Lys-C, Lys-N, and chymotrypsin. In general, these enzymes differ by their specificity for cleaving the amide bond before or after a specific residue. For complex protein samples, a combination of highly selective proteases has proved to increase proteome coverage by creating complementary peptides (13). [Pg.390]

A comprehensive proteomics approach has been developed to identify the components of phosphoprotein complexes and includes the following experimental steps isolation of native phosphoproteins and associated proteins by affinity chromatography, ID PAGE separation of the affinity-isolated proteins, proteolysis of the protein spots, LC-ESI-MS/MS analysis of the cleaved peptides, and database searching for indentification of the proteins involved in the complex formation.178... [Pg.491]

See other pages where Proteomics analysis cleave proteins is mentioned: [Pg.210]    [Pg.379]    [Pg.80]    [Pg.201]    [Pg.272]    [Pg.463]    [Pg.390]    [Pg.94]    [Pg.81]    [Pg.259]    [Pg.1343]    [Pg.332]    [Pg.168]    [Pg.252]    [Pg.524]    [Pg.639]    [Pg.4]    [Pg.12]    [Pg.493]    [Pg.342]    [Pg.113]    [Pg.788]    [Pg.615]    [Pg.628]    [Pg.276]   


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2- cleaved

Cleave

Protein analysis

Proteome analysis

Proteome/Proteomic analysis

Proteomic analysis

Proteomic analysis proteins

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