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Classification of methods

Based on the molar absorptivities of the products, the glucose oxidase-peroxidase assay may be expected to yield higher sensitivity and a lower detection limit. [Pg.45]

Ideally, the indicator enzyme converts primary product into measured product in a linear manner, meaning that every molecule of primary product is instantaneously converted, regardless of substrate concentration. To accomplish this, primary product concentrations are kept low, so that they fall into the linear region of the saturation kinetics curve. For linear conversion at all analyte (primary substrate) concentrations, the effective rate of the indicator reaction, (Feff)ind must equal ymax for the primary reaction  [Pg.45]

Since KmPi is a characteristic of the indicator enzyme, and [PI] is dictated by the analyte concentration, the only variables that can be controlled experimentally are ( V/maX) nd, which is equal to kCM [Eln il (cf. Chapter 2) and the cosubstrate concentration [S2]. For this reason, a large excess (100-fold or more) of indicator enzyme is employed, in addition to saturating levels of cosubstrate (0-dianisidine in Eq. 3.8, and NADP+ in Eq. 3.10). [Pg.45]

When making initial rate measurements with coupled enzyme systems, there is often a significant lag time during which the linkage products build up to steady-state concentrations.6 Remember that some dehydrogenase reactions possess unfavorable equilibria lactate dehydrogenase, for example, which catalyzes the reaction shown in Eq. 3.12  [Pg.45]

Enzymatic assay methods are classified as fixed-time assays, fixed-change assays, or kinetic (initial rate) assays. Kinetic assays continuously monitor concentration as a function of time pseudo-first-order conditions generally apply up to 10% completion of the reaction to allow the initial reaction rate to be determined. If the initial substrate concentration is 10Km, then the initial rate is directly proportional to enzyme concentration. At low initial substrate concentrations ( 0.1 Km), the initial rate will be directly proportional to initial substrate concentration (cf. Chapter 2). For enzyme quantitation, a plot of initial rate against [E] provides a linear [Pg.45]

O Table 63.3 lists the four classes of methods and instruments implemented for safeguards verification measurements. [Pg.2903]

HM methods can be classified in three ways either (1) according to their objective (analysis or exploration of reaction mechanism), (2) the nature [Pg.373]

The basic data gathering methods are direct methods which allow visual inspection or at least direct measurement of properties, and indirect methods whereby we infer reservoir parameters from a number of measurements taken in a borehole. The main techniques available within these categories are summarised in the following table  [Pg.125]

This section will look at formation and fluid data gathering before significant amounts of fluid have been produced hence describing how the static reservoir is sampled. Data gathered prior to production provides vital information, used to predict reservoir behaviour under dynamic conditions. Without this baseline data no meaningful reservoir simulation can be carried out. The other major benefit of data gathered at initial reservoir conditions is that pressure and fluid distribution are in equilibrium this is usuaily not the case once production commences. Data gathered at initial conditions is therefore not complicated [Pg.125]


See also Spectrophotometry Colour change interval 264 Colour measurement classification of methods, 651... [Pg.859]

The classification of methods for studying electrode kinetics is based on the criterion of whether the electrical potential or the current density is controlled. The other variable, which is then a function of time, is determined by the electrode process. Obviously, for a steady-state process, these two quantities are interdependent and further classification is unnecessary. Techniques employing a small periodic perturbation of the system by current or potential oscillations with a small amplitude will be classified separately. [Pg.304]

Table 25. Classification of methods of dye and textile effluent treatment [314]... Table 25. Classification of methods of dye and textile effluent treatment [314]...
Classification of methods for simultaneous determination of anions and cations by IC... [Pg.1213]

Our emphasis in this book is not so much on the subtleties that distinguish one historical name from another, or one result from another. The basic classification of methods described in Chapter 7, for example, is founded on the arrangements of the fundamental forces and flows that give rise to separation and ultimately determine their efficacy. It is the belief of this author that this approach is ultimately the most lasting one it will... [Pg.13]

Basic Approaches to Separation. Analysis and Classification of Methods According to Underlying Transport Characteristics, J. C. Giddings, Sep. Sci. Technol., 13, 3... [Pg.299]

The selection of an analytical method may also be influenced by external regulatory requirements or by special requests of the customer. The following terms often appear in the analytical jargon and were compiled by Garfield [8] the classification of methods relies on purpose of the method or the administrative background. [Pg.14]

Another classification of methods for measuring anisotropies is that of partially oriented molecules, the most important examples of which are the liquid crystals. The utility of this method lies in the fact that many molecules can be dissolved in liquid crystals without destroying the liquid crystal phase.12-15 The solute molecules are able to translate relatively freely in one direction-along the optic axis. As a result of this motion the intermolecular dipole-dipole interactions are averaged to zero but the intramolecular interactions are not. Also since the solute molecules cannot rotate equally in all directions, there will be an anisotropic contribution to the observed shielding. [Pg.488]

Classification of Methods for Immobilizing (or Heterogenizing) Homogeneous Catalysts... [Pg.756]

Table 1.1. Classification of methods for photochemical water decomposition... Table 1.1. Classification of methods for photochemical water decomposition...
A common classification of methods for the size enlargement of particulate solids distinguishes between two types of process (Chapter 5),... [Pg.890]


See other pages where Classification of methods is mentioned: [Pg.125]    [Pg.651]    [Pg.651]    [Pg.653]    [Pg.896]    [Pg.54]    [Pg.243]    [Pg.245]    [Pg.766]    [Pg.3]    [Pg.672]    [Pg.62]    [Pg.528]    [Pg.5]    [Pg.191]    [Pg.373]    [Pg.373]    [Pg.693]    [Pg.83]    [Pg.87]    [Pg.45]    [Pg.45]    [Pg.155]    [Pg.612]   
See also in sourсe #XX -- [ Pg.13 , Pg.14 , Pg.15 ]




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Classification of Enzymatic Assay Methods

Classification of Fault Detection Methods

Classification of MCDA Methods

Classification of MCMP Methods

Classification of Methods For Regulatory Purposes

Classification of Pressurization Methods

Classification of Solvents using Multivariate Statistical Methods

Classification of chromatographic methods

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