Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Displacement chromatography

Suresh, V., Gallant, S., and Cramer, S., Immobilized metal affinity chromatography displacer characteristics of traditional mobile phase modifiers, Biotechnol. Prog., 12, 84, 1996. [Pg.127]

New concepts presented in this edition include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, and capillary electromigration techniques. The book also discusses LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, field-flow fractionation, retention models for ions, and polymer HPLC. [Pg.696]

In two-way chromatography displacement effects are exploited as wellt. Here, there is no addition of a displacing species. By alternating the direction of flow, the more retained species in the feed serve as the displacer for the less retained species. Two-way chromatography may lead to an elevation of the concentration. However, a serious drawback is that it is far too complex for separation of a multi-component mixture. [Pg.91]

Displacement chromatography is one of the three basic modes of chromatographic operation, the oAict two being frontal analysis and elution chromatography. Displacement chromatography is rarely, if ever, used for analytical separations, but is usefid for preparative separations. It has also been used for trace enrichment... [Pg.617]

Both gas-solid and gas-liquid chromatography may inyolve the following techniques elution chromatography, displacement chromatography, frontal chromatography, and vacancy chromatography. [Pg.14]

The ftxed-bed processes studied so far are generally useful for separating one solute or one ionic species from the solvent in some cases, more than one solute or one ionic species are also separated from the solvent Separation of each individual species present in a multicomponent gas mixture or liquid solution can be achieved in a fixed bed of adsorbent particles if the mode of operation, e.g. the method of feed introduction, is changed from that used so far. A number of methods are commonly used to this end. They are elution chromatography, displacement chromatography and firontal chromatography (see Figure 7.1.5(c)). [Pg.527]

Development of the Chromatogram. The term development describes the process of performing a chromatographic separation. There are several ways in which separation may be made to occur, eg, frontal, displacement, and elution chromatography. Frontal chromatography uses a large quantity of sample and is usually unsuited to analytical procedures. In displacement and elution chromatography, much smaller amounts of material are used. [Pg.105]

Both frontal and displacement chromatographies suffer a significant disadvantage in that once a column has been used, part of the sample remains on the column. The column must be regenerated before reuse. In elution chromatography all of the sample material is usually removed from the column... [Pg.105]

Modes of Operation The classical modes of operation of chromatography as enunciated by Tisehus [Kolloid Z., 105, 101 (1943)] are elution chromatography, frontal analysis, and displacement development. Basic features of these techniques are illustrated in Fig. [Pg.1530]

Displacement chromatography is suitable for the separation of multicomponent bulk mixtures. For dilute multicomponent mixtures it allows a simultaneous separation and concentration. Thus, it permits the separation of compounds with extremely low separation fac tors without the excessive dilutiou that would be obtained in elution techniques. [Pg.1531]

FIG. 16-30 Operational steps in displacement chromatography, The column, initially equilibrated with a carrier solvent at time 0, is loaded with feed until time tp and supplied with displacer for a time to + tp. Development of the displacement train occurs during the time to and elution of the separated products ends at time tp. tp is the time required to remove the displacer from the column and restore the initial conditions, Components are niimhered in order of decreasing affinity for the stationary phase, [Reference Horoath et at, J, Ghromatogr, 2i8, 365 (1981). Reprinted with peimission of], Ghromatogr,]... [Pg.1531]

Displacement Development A complete prediction of displacement chromatography accounting for rate factors requires a numerical solution since the adsorption equilibrium is nonlinear and intrinsically competitive. When the column efficiency is high, however, useful predictious can be obtained with the local equilibrium theoiy (see Fixed Bed Transitions ). [Pg.1536]

TABLE 16-15 Concentrations and h-Function Roots for Displacement Chromatography of a Mixture of M-1 Components Numbered in Order of Decreasing Affinity for the Stationary Phase (Adapted from Frenz and Horvath/ 1985). [Pg.1537]

Example 12 Calculation of Band Profiles in Displacement Chromatography Au equimolar mixture of two compoueuts (couceutra-tious cf = = 1 arbitrary iiuit) is separated with a displacer with couceutratiou... [Pg.1537]

FIG. 16 36 Dimensionless time-distance plot for the displacement chromatography of a binary mixture. The darker lines indicate self-sharpening boundaries and the thinner lines diffuse boundaries. Circled numerals indicate the root number. Concentration profiles are shown at intermediate dimensionless column lengths = 0.43 and = 0.765. The profiles remain unchanged for longer column lengths. [Pg.1538]

FIG. 16"37 Schematic showing the intersection of the operating line with the pure-component isotherms in displacement chromatography. Conditions are the same as in Fig. 16-36. [Pg.1539]

Chromatography Chromatography is a sorptive separation technique that allows multicomponent separations in both gas and Hquid phase. As a preparative tool, it is often used as a displacement-purge process, although many applications employ an inert-displacement mode, especially for use in analysis. General characteristics and operating modes are discussed in a separate part of this section. [Pg.1544]

Isolation procedures for many biochemicals are based on chromatography. Practically any substance can be selected from a crude mixture and eluted at relatively high purity from a chromatographic column with the right combination of adsorbent, conditions, and eluant. For bench scale or for a small pilot plant, such chromatography has rendered alternate procedures such as electrophoresis nearly obsolete. Unfortunately, as size increases, dispersion in the column ruins resolution. To produce small amounts or up to tens of kilograms per year, chromatography is an excellent choice. When the scale-up problem is solved, these procedures should displace some of the conventional steps in the chemical process industries. [Pg.2144]

Conventional elution chromatography has the serious disadvantage of dilution, and usually a concentration step must follow. The technique of displacement chromatography circumvents dilution and may even result in an eluant more concentrated than the feed. A displacer compound breaks the desired product from the chromatographic material sharply, and a column heavily loaded with several biochemicals will release them one at a time depending on their adsorption equilibria. However, the displacers tena to be expensive and can be troublesome to remove from the product. [Pg.2144]

A chromatographic separation can be developed in three ways, by displacement development, by frontal analysis, and by elution development, the last being almost universally used in all analytical chromatography. Nevertheless, for the sake of completeness, and because in preparative chromatography (under certain conditions of mass overload) displacement effects occur to varying extents, all three development processes will be described. [Pg.7]


See other pages where Displacement chromatography is mentioned: [Pg.112]    [Pg.257]    [Pg.533]    [Pg.477]    [Pg.461]    [Pg.1081]    [Pg.1089]    [Pg.112]    [Pg.257]    [Pg.533]    [Pg.477]    [Pg.461]    [Pg.1081]    [Pg.1089]    [Pg.590]    [Pg.337]    [Pg.48]    [Pg.340]    [Pg.149]    [Pg.536]    [Pg.105]    [Pg.105]    [Pg.1530]    [Pg.1531]    [Pg.1537]    [Pg.1539]    [Pg.1554]    [Pg.1555]    [Pg.1555]    [Pg.2063]    [Pg.337]    [Pg.514]    [Pg.8]    [Pg.17]    [Pg.492]    [Pg.62]    [Pg.82]   
See also in sourсe #XX -- [ Pg.112 , Pg.132 ]

See also in sourсe #XX -- [ Pg.938 , Pg.939 , Pg.959 , Pg.960 , Pg.961 ]

See also in sourсe #XX -- [ Pg.161 ]




SEARCH



Alternative Modes of Displacement Chromatography

Applications of Displacement Chromatography

Band Profiles in Displacement Chromatography with the Ideal Model

Boundary conditions displacement chromatography

Carrier solution, displacement chromatography

Chromatographic processes displacement chromatography

Chromatography displacement development

Column equilibration, displacement chromatography

Development for Displacement Chromatography

Dispersion Displacement chromatography

Displacement Chromatography with a Nonideal Column

Displacement and Multiple Column Partition Chromatography

Displacement chromatography adsorbents

Displacement chromatography advantages

Displacement chromatography alternative modes

Displacement chromatography calculations

Displacement chromatography chromatographic separation

Displacement chromatography complex mixture separation

Displacement chromatography developing

Displacement chromatography displacer concentration

Displacement chromatography displacer, definition

Displacement chromatography gradient elution

Displacement chromatography methods development

Displacement chromatography mobile phases

Displacement chromatography peptide

Displacement chromatography preparative technique

Displacement chromatography principles

Displacement chromatography protein

Displacement chromatography sample

Displacement chromatography schematic

Displacement chromatography selective

Displacement chromatography shock layers

Displacement chromatography trace component enrichment

Displacement chromatography, description

Elution modified displacement chromatography

Equilibrium-dispersive model displacement chromatography

Optimization displacement chromatography

Preparative-scale chromatography displacement

Purification of Proteins by Displacement Chromatography

Selection, displacement chromatography

Self-displacement chromatography

Separation selectivity, displacement chromatography

© 2024 chempedia.info