Column equilibration, displacement chromatography

FIG. 16-30 Operational steps in displacement chromatography, The column, initially equilibrated with a carrier solvent at time 0, is loaded with feed until time tp and supplied with displacer for a time to + tp. Development of the displacement train occurs during the time to and elution of the separated products ends at time tp. tp is the time required to remove the displacer from the column and restore the initial conditions, Components are niimhered in order of decreasing affinity for the stationary phase, [Reference Horoath et at, J, Ghromatogr, 2i8, 365 (1981). Reprinted with peimission of], Ghromatogr,]... 

Displacement chromatography is characterized by the introduction of a discrete volume of sample into the chromatographic column that has been previously equilibrated with a weak mobile phase, termed the carrier. This carrier is chosen so that the individual components of the sample (the solutes) are significantly retained by the stationary phase. The displacement is accomplished by following the sample with a new mobile phase containing some concentration of the displacer, a molecule with a higher affinity for the stationary phase than that of any of the solutes. The solutes are displaced from the stationary phase by the higher-... 

In displacement chromatography the column is first equilibrated with mobile phase (referred to as the carrier), chosen for its low solvent strength in the separation system. 

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