Displacement and Multiple Column Partition Chromatography

Stopcocks are used to control the flow rate and should be wide bore to prevent plugging with viscous mobile phases. Removable bottoms are used for those separations that require long times. The bottom can be removed and the column packing pushed out with a plunger (CARE this is not as easy as it sounds). The column packing then can be sectioned, and the desired compounds extracted or washed from the inert phase for further use. [Pg.155]

In Order from Most Active to Least Active [Pg.155]

In Order from Least to Greatest Adsorption by the Column Adsorbent [Pg.155]

The main equations required to solve several column chromatography problems are presented below and in the example calculations. These equations are based upon a liquid-liquid partition process rather than an adsorption process and are usually valid only for processes involving very dilute systems. [Pg.156]

For the most part, the mathematical problems involved here concern (1) how much mobile phase is necessary for a separation, (2) when a mixture is actually separated, and (3) when each component comes through the column. The basic terminology used in this connection is [Pg.156]

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