Displacement chromatography trace component enrichment

FIGURE 11.13 Separation of a hypothetical mixture containing trace component 3 by elution (panel A) or displacement (panel B) chromatography. Enrichment of 3 and 4 relative to 1 and 2 in B is shown. (Reprinted with permission from Elsevier from Viscomi, G.C. et ah, J. Chromatogr., 440, 157, 1988. Copyright.)... 

As outlined above displacement chromatography may find its most important uses in the analytical area. The ability to enrich trace levels of components is ideally suited to the proteomics where more powerful tools are desperately needed to address the vast concentration ranges present in order to identify trace components. The technique also offers a way to isolate large quantities of protein variants which is important for the identification and characterization of minor product-related impurities commonly associated with therapeutic proteins. 

---