Displacement chromatography alternative modes

Displacement chromatography offers an attractive alternative to the elution mode of operation for preparative purification (S. Cramer, personal communication, 1999). Further investigations must be carried out to identify more cost-effective, nontoxic, and readily detectable displacers that are commercially available to the biotechnology industries. Displacers with high affinities in a range of commercially available stationary phases must be identified to facilitate the development of displacement steps on these materials. This will require significant advances in our understanding of the nature of affinity of these systems. 

Displacement chromatography is seen primarily as an alternative to overloaded elution chromatography. The advantages of this mode are as follows (1) concentrated product pools are obtained (2) the stationary phase is utilized more effectively (3) large... 

---