Purification of Proteins by Displacement Chromatography

FIGURE 3 Displacement chromatography of /3-lactoglobulins at 1440 cm / hr. Column 100 X 4.6 mm POROS 10 HQ (10 /z particles) mobile phase 10 mM Tris-HCI, pH 7.0 displacer 12 mg/mL heparin in carrier feed 60 mg /3-lactoglobulin crude mixture fraction volume 200 /nL (from Gerstner et a/.,32 with permission from Elsevier Science). 

TABLE I High Molecular Weight Displacers Employed for the Ion-Exchange Displacement Chromatography of Proteins 

DEAE Sephadex A50 and DEAE Sephacel (weak anion exchange) DEAE cellulose Carboxymethyld extrans Ovalbumin, /J-lactoglobulins A and B serum proteins RNA polymerase II 15-17 

CM cellulose Ampholine (pH 4-6) Human serum albumin, a-fetoprotein 35 

TSK DEAE 5PW (weak anion exchange, 5 fi particles) Chondroitin sulfate and polygalactouronic acid /j-lactoglobulins A and B 19 

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