Cell growth tests

Disks (0 1.5 cm for cell growth tests or 0 0.5 cm for ELISA and I-HFn tests) were cut from the sheets, extracted in ethanol (6h, r.t.) and then incubated overnight at 37°C in Phosphate Buffered Solution (PBS, pH=7.4). 

Initially, the cytotoxicity against chick embryo fibroblasts of BPA, tyrosine, tyrosine dipeptide, and the dipeptide derivatives used in the synthesis of the polymers shown in Fig. 7 were evaluated in a comparative experiment (43). The surface of standard tissue culture wells was coated with 5 mg of each test substance. Then the adhesion and proliferation of the fibroblasts was followed over a 7-day period. Among all test substances, BPA was clearly the most cytotoxic material. Monomeric tyrosine derivatives containing the ben-zyloxycarbonyl group were also cytotoxic, while tyrosine itself, tyrosine dipeptide, and most of the protected dipeptide derivatives did not noticeably interfere with cell growth and adhesion and were therefore classified on a preliminary basis as possibly "nontoxic."... 

Peter and Wang [266] invented a channel flow cell for rapid growth of CdTe films they showed that 2 p,m Aims can be deposited in less than 20 min, as opposed to the 2-3 h normally required in the conventional stirred single batch cells. The as-deposited films were structurally more disordered than the conventional ones, but after annealing and type conversion they became suitable for fabrication of efficient solar cells. A test cell with an AMI.5 efficiency approaching 6% was fabricated using a film prepared in the channel cell. 

C. Kotts, E. Gilkerson, D. Trinh, G. Hawker, A. Chen, H. Gazzano-Santoro, Assay validation report Cell proliferation test for human growth hormone, Pharmacopeial Forum, 25(3), 8313 (1999). 

Radical polymerization of maleic anhydride and fullerene was used to obtain a new material, the photodynamic properties of which have been studied in vitro and in vivo. HeLa and bone tumor cell growth were inhibited by treatment with fullerene and light, so the polymer was tested on mice affected by bone tumor. After injection and irradiation, tumor size and weight were reduced and the mouse survival time was extended (Jiang and Li, 2007). The photodynamic properties of a supramolecular cucurbit[8]uril-fullerene complex have been studied by the same authors (Jiang and Li, 2006) who attributed HeLa cell death mainly to the damage of membrane phosphohpids and proteins. 

This cell line was recently established by Mohan et al. [64], Intensive testing of this cell line regarding its suitability for pharmaceutical purposes has not been performed yet. The cell line is highly interesting, however, because of its controlled cell growth and differentiation capabilities. An integrated DNA section allows cell growth to be switched on or off. Therefore, further examination of this cell line should be of major interest and importance for various pharmaceutical researches. 

A transmissible sarcoma of the chicken has been under observation in this laboratory for the past fourteen months, and it has assumed of late a special interest because of its extreme malignancy and a tendency to widespread metastasis. In a careful study of the growth, tests have been made to determine whether it can be transmitted by a filtrate free of the tumour cells. Attempts to so transmit rat, mouse, and dog tumours have never succeeded and it was supposed that the sarcoma of the fowl would not differ from them in this regard, since it is a typical neoplasm. On the contrary, small quantities of a cell-free filtrate have sufficed to transmit the growth to susceptible fowls. 

The same group has used a closely related strategy to synthesize all eight stereoisomers of preussin by non-stereoselective reactions. They tested the bioactivities of the preussin isomers against cell growth of the fission yeast cdc25 mutant and found, that all stereoisomers were almost equally bioactive [55]. 

Assessing the condition of the cells ahead of time can save valuable reagents. Things to note include well contamination, empty wells, uneven volume in wells, and uneven cell growth. Cells in the test plate should look healthy and the confluency should be between 70 and 90%. 

In addition to the above-outlined cytotoxic effects of gemcitabine, it also serves to act as a radiation sensitizer in many cell lines (see Table 1). Depending on the cell line tested, the drug concentration, the schedule of administration, and the cell proliferative status (e.g., plateau vs exponential growth), relative enhancement ratios (RERs) in the range of 1.1-2.5 have been reported. There is no evidence that preferential radiosensitization occurs in more radioresistant cell lines. 

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